Cortex glia clear dead young neurons via Drpr/dCed-6/Shark and Crk/Mbc/dCed-12 signaling pathways in the developing Drosophila optic lobe
2019; Elsevier BV; Volume: 453; Issue: 1 Linguagem: Inglês
10.1016/j.ydbio.2019.05.003
ISSN1095-564X
AutoresRyosuke Nakano, Masashi Iwamura, Akiko Obikawa, Yu Togane, Yusuke Hara, T Fukuhara, Masatoshi Tomaru, Toshiyuki Takano‐Shimizu, Hidenobu Tsujimura,
Tópico(s)Retinal Development and Disorders
ResumoThe molecular and cellular mechanism for clearance of dead neurons was explored in the developing Drosophila optic lobe. During development of the optic lobe, many neural cells die through apoptosis, and corpses are immediately removed in the early pupal stage. Most of the cells that die in the optic lobe are young neurons that have not extended neurites. In this study, we showed that clearance was carried out by cortex glia via a phagocytosis receptor, Draper (Drpr). drpr expression in cortex glia from the second instar larval to early pupal stages was required and sufficient for clearance. Drpr that was expressed in other subtypes of glia did not mediate clearance. Shark and Ced-6 mediated clearance of Drpr. The Crk/Mbc/dCed-12 pathway was partially involved in clearance, but the role was minor. Suppression of the function of Pretaporter, CaBP1 and phosphatidylserine delayed clearance, suggesting a possibility for these molecules to function as Drpr ligands in the developing optic lobe.
Referência(s)