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Mammaglobin in peripheral blood and tumor in breast cancer patients

2016; Volume: 62; Issue: 4 Linguagem: Inglês

10.18097/pbmc20166204453

2310-6972

K. V. Bozhenko, Н. В. Харченко, Е. Ф. Васкевич, Е. А. Кудинова, A. V. Oorzhak, Rozhkova Ni, И. Д. Троценко,

Estrogen and related hormone effects

Currently, no molecular biological markers do exist for early diagnosis of breast cancer. One of the possible candidates for the marker of early breast cancer is mammaglobin (MGB1) or SCGB2A2 (secretoglobin, family 2A, member 2), characterized by the maximal expression level in early breast cancer. Using the RT-PCR method MGB1 mRNA expression was examined in 57 tumor tissue samples and 57 samples of morphologically non-malignant tissue (MNT) of breast cancer (BC) patients. Specificity and sensitivity of the MGB1 mRNA assay in peripheral blood of BC patients was evaluated by nested PCR. 169 blood samples (from 95 BC patients, 22 from patients with benign breast tumors, 28 from patients with tumors of other localizations, and 24 samples from healthy donors) have been analyzed. MGB1 expression was significantly higher in BC tissue samples compared to MNT (p=0.0019). The maximal expression level was in the samples T1 (p=0.013), stage I BC (p=0.037), GI (p=0.0019). The MGB1 expression positively correlated with expression of estrogen (p = 0,034) and progesterone (p=0.0004) receptors. Sensitivity and specificity of the MGB1 mRNA assay in peripheral blood were 60.6% and 92.3%, respectively. Expression of MGB1 was higher in BC than MNT and it decreased during BC progression. The sensitivity and specificity of the MGB1 mRNA assay may be used as an additional diagnostic method. V nastoiashchee vremia ne sushchestvuet molekuliarno-biologicheskikh markerov dlia diagnostiki rannego raka molochnoĭ zhelezy (RMZh). V kachestve vozmozhnogo markera rassmatrivaetsia mammaglobin (hMAM), uroven' ékspressii kotorogo maksimalen pri rannem RMZh. C pomoshch'iu metoda OT-PTsR opredelen uroven' ékspressii mRNK mammaglobina v 57 parnykh obraztsakh opukholevoĭ i morfologicheski neizmenennoĭ tkani (MNT) molochnoĭ zhelezy, poluchennykh ot bol'nykh RMZh. Otsenena spetsifichnost' i chuvstvitel'nost' analiza mRNK hMAM v perifericheskoĭ krovi bol'nykh RMZh metodom gnezdnoĭ PTsR. Proanalizirovano 169 obraztsov krovi (95 ot bol'nykh RMZh, 22 ot bol'nykh s dobrokachestvennymi opukholiami molochnoĭ zhelezy, 28 ot bol'nykh s opukholiami drugoĭ lokalizatsii i 24 obraztsa zdorovykh donorov). Ékspressiia hMAM byla znachitel'no vyshe v obraztsakh tkani RMZh po sravneniiu s MNT (r=0,0019). Maksimal'nyĭ uroven' ékspressii otmechen v obraztsakh T1 (r=0,013), stadii I (r=0,037), GI (r=0,0019). Vyiavlena polozhitel'naia korreliatsiia mezhdu ékspressieĭ hMAM i retseptorov éstrogena (p=0,034) i progesterona (r=0,0004). Chuvstvitel'nost' i spetsifichnost' opredeleniia ékspressii hMAM v perifericheskoĭ krovi sostavila 60,6 i 92,3% sootvetstvenno. Ékspressiia hMAM pri RMZh byla vyshe, chem v MNT i snizhalas' pri vysokoĭ rasprostranennosti zabolevaniia. mRNK mammaglobina obnaruzhena v 61% obraztsov krovi bol'nykh RMZh, v 22,7% – pri dobrokachestvennykh obrazovaniiakh molochnoĭ zhelezy i lish' v odnom obraztse krovi pri drugoĭ lokalizatsii opukholevogo protsessa – u bol'noĭ rakom legkogo. V obraztsakh krovi zdorovykh donorov mRNK mammaglobina ne obnaruzhena. Takim obrazom, opredelenie mRNK hMAM v perifericheskoĭ krovi, po-vidimomu, mozhno rassmatrivat' kak dopolnitel'nyĭ metod diagnostiki RMZh.