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Antifungal effects of the flavonoids kaempferol and quercetin: a possible alternative for the control of fungal biofilms

2019; Taylor & Francis; Volume: 35; Issue: 3 Linguagem: Inglês

10.1080/08927014.2019.1604948

1029-2454

Marcos Fábio Gadelha Rocha, Jamille Alencar Sales, Maria Gleiciane da Rocha, Livia Maria Galdino, Lara de Aguiar, Waldemiro de Aquino Pereira‐Neto, Rossana de Aguiar Cordeiro, Débora de Souza Collares Maia Castelo‐Branco, José Júlio Costa Sidrim, Raimunda Sâmia Nogueira Brilhante,

Gut microbiota and health

This study aimed to determine the minimum inhibitory concentration (MIC) of kaempferol and quercetin against planktonic and biofilm forms of the Candida parapsilosis complex. Initially, nine C. parapsilosis sensu stricto, nine C. orthopsilosis and nine C. metapsilosis strains were used. Planktonic susceptibility to kaempferol and quercetin was assessed. Growing and mature biofilms were then exposed to the flavonoids at MIC or 10xMIC, respectively, and theywere also analyzed by confocal laser scanning microscopy. The MIC ranges were 32-128 µg ml−1 for kaempferol and 0.5-16 µg ml−1 for quercetin. Kaempferol and quercetin decreased (P < 0.05) the metabolic activity and biomass of growing biofilms of the C. parapsilosis complex. As for mature biofilms, the metabolic effects of the flavonoids varied, according to the cryptic species, but kaempferol caused an overall reduction in biofilm biomass. Microscopic analyses showed restructuring of biofilms after flavonoid exposure. These results highlight the potential use of these compounds as sustainable resources for the control of fungal biofilms.