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Effect of different concentrations of l-carnitine in extender for semen cryopreservation in sheep

2019; Elsevier BV; Volume: 89; Linguagem: Inglês

10.1016/j.cryobiol.2019.05.009

1090-2392

Clara Vieira de Souza, Felipe Zandonadi Brandão, Juliana Dantas Rodrigues Santos, Vivian Angélico Pereira Alfradique, Vanessa Moreira Barbosa dos Santos, Maria Clara da Cruz Morais, Paulo Sérgio Cerqueira Rangel, Andreza Amaral da Silva, Joanna Maria Gonçalves Souza‐Fabjan,

Reproductive Biology and Fertility

This study assessed the effect of l-carnitine (LC) in sheep semen extenders containing or not egg yolk for cryopreservation in sheep. Two extenders (TRIS-egg yolk or the commercial optiXcell™ IMV medium) were used, totaling six groups: IMV - (0, 5 and 10 mM LC) and TRIS - (0, 5 and 10 mM LC). After the freezing-thawing process and throughout incubation at 38 °C for up to 3 h, several parameters were evaluated: sperm kinetics, hypoosmotic, plasma membrane integrity, capacitation status and lipid peroxidation level. The supplementation of either 5 or 10 mM LC randomly affected some parameters and, overall, TRIS was superior (P < 0.05) than IMV extender. In the LC-groups, IMV had greater (P < 0.05) oxidative stress than TRIS. In conclusion, although LC affected isolated parameters, its supplementation in semen extender had no consistently beneficial effect on freezing-thawing ram sperm.