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Albumin In Situ Hybridization Can Be Positive in Adenocarcinomas and Other Tumors From Diverse Sites

2019; Oxford University Press; Volume: 152; Issue: 2 Linguagem: Inglês

10.1093/ajcp/aqz032

1943-7722

Aqsa Nasir, Heidi D. Lehrke, Taofic Mounajjed, Samar M. Said, Lizhi Zhang, Saba Yasir, Sejal Shah, Vishal S. Chandan, Thomas C. Smyrk, Roger K. Moreira, Jennifer M. Boland Froemming, Loren P. Herrera Hernandez, Tsung‐Teh Wu, Rondell P. Graham,

Pancreatic and Hepatic Oncology Research

Albumin messenger RNA (mRNA) expression is a marker of hepatocellular differentiation. Most published data are from review of tissue microarrays, and albumin in situ hybridization (ISH) expression across several tumor types is incompletely characterized.Sections from 221 tumors were evaluated for albumin mRNA. Immunohistochemistry was used to confirm diagnoses. Albumin ISH was performed according to manufacturer-provided instructions. Fifty-nine cases were evaluated with both commercial ISH assays.Albumin mRNA was detected in all hepatocellular carcinomas (HCCs) and 81% of intrahepatic cholangiocarcinomas. Lung (20%), gallbladder (39%), hepatoid pancreatic (n = 1 of 1) adenocarcinoma, breast invasive ductal carcinoma (18%), yolk sac tumor (25%), and acinar cell carcinoma (29%) showed expression. Both assays were concordant in 93% of cases.Albumin ISH was expressed in all HCCs studied. It was also positive in intrahepatic cholangiocarcinoma and patchy positive in gallbladder adenocarcinoma and a subset of other neoplasms, which can be a potential pitfall.