Sequence diversity and in silico structure prediction of the vacuolar invertase inhibitor gene from potato (Solanum tuberosum L.) cultivars differing in sugar content
2019; Elsevier BV; Volume: 295; Linguagem: Inglês
10.1016/j.foodchem.2019.05.096
ISSN1873-7072
AutoresSagar Datir, Duhita Mirikar, Ameeta RaviKumar,
Tópico(s)Microbial Metabolites in Food Biotechnology
ResumoThe aim of this study was to examine the variations in sugar content and identify the polymorphism in vacuolar invertase inhibitor (INH2) gene from Indian non-processing (Kufri Jyoti, Kufri Pukhraj and PU1) and exotic processing (Atlantic and Frito Lay-1533) potato cultivars. Upon cold storage (4 °C) processing cultivars maintained low reducing sugars as compared to non-processing cultivars. Sequencing of the INH2 gene identified four alleles of which three identified as novel alleles. A total twelve SNPs resulted in silent mutations, with five conferring the amino acid substitutions. Phylogenetic analysis suggests a highly conserved nature of the INH2 gene. The 3D predicted structures generated for all the alleles revealed slight variations in the orientation of the helices (α1-3) in N-terminal region. Sequence polymorphism observed in INH2 alleles in processing and non-processing potato cultivars can be correlated with the observed variations in the sugar content suggesting a possible role in cold-induced sweetening.
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