Portal de Periódicos da CAPES

First Report of Corynespora Leaf Spot of Cotton Caused by Corynespora cassiicola in Central India

2019; American Phytopathological Society; Volume: 103; Issue: 7 Linguagem: Inglês

10.1094/pdis-05-18-0823-pdn

1943-7692

V. N. Salunkhe, S. P. Gawande, Dipak T. Nagrale, Neelakanth S. Hiremani, Nandini Gokte‐Narkhedkar, V. N. Waghmare,

Banana Cultivation and Research

HomePlant DiseaseVol. 103, No. 7First Report of Corynespora Leaf Spot of Cotton Caused by Corynespora cassiicola in Central India PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Corynespora Leaf Spot of Cotton Caused by Corynespora cassiicola in Central IndiaV. N. Salunkhe, S. P. Gawande, D. T. Nagrale, N. S. Hiremani, N. Gokte-Narkhedkar, and V. N. WaghmareV. N. Salunkhe†Corresponding author: V. N. Salunkhe; E-mail Address: vanita.salunkhe@icar.gov.inhttp://orcid.org/0000-0003-3123-5534ICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, IndiaSearch for more papers by this author, S. P. GawandeICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, IndiaSearch for more papers by this author, D. T. NagraleICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, IndiaSearch for more papers by this author, N. S. HiremaniICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, IndiaSearch for more papers by this author, N. Gokte-NarkhedkarICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, IndiaSearch for more papers by this author, and V. N. WaghmareICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, IndiaSearch for more papers by this authorAffiliationsAuthors and Affiliations V. N. Salunkhe † S. P. Gawande D. T. Nagrale N. S. Hiremani N. Gokte-Narkhedkar V. N. Waghmare ICAR-Central Institute for Cotton Research, Nagpur 440010, Maharashtra, India Published Online:20 May 2019https://doi.org/10.1094/PDIS-05-18-0823-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In 2017, an unusual leaf spot on cotton (Gossypium hirsutum L.) was recorded from the Nagpur district, Maharashtra state, India. The unknown leaf spot was responsible for 30 to 40% premature defoliation in cultivar PKV-081, BG-II hybrids, and cultivar Suraj. The disease was first observed in 10- to 12-leaf-stage cotton and continued until harvest. In the first week of September, disease symptoms were observed in the lower canopy, which progressed upward to cover the entire plant by mid-September. Initially, leaves exhibited circular to irregular, dark red, small, numerous lesions, which over time became brown (5 to 10 mm) and surrounded by a dark border. As lesions matured, alternating rings of light and dark brown bands developed. The most mature lesions presented a target-type appearance. Diseased leaf sections (5 mm2) were cut from the lesion edge and surface disinfested with 1% NaClO for 2 min, rinsed in sterile water, blotted dry with sterile blotter paper, and plated on Sabouraud dextrose agar. Plates were incubated at 25 ± 2°C for 5 days under a 12-h photoperiod. Purified colonies exhibited white to dark gray, profuse mycelial growth. Conidiophores were pale brown, simple, cylindrical, and septate with intermittent branching. Conidia were subhyaline to olivaceous, solitary or in chains, straight to slightly curved, obclavate to cylindrical, 30 to 128 × 5 to 12 µm, with one to 17 pseudosepta. Morphological characteristics were consistent with the original descriptions of Corynespora cassiicola on cotton (Jones 1961) and more recent reports (Butler et al. 2016; Conner et al. 2013). For pathogenicity, three to four true leaf stage leaves were detached and gently washed. Centrifuge tubes (15 ml) with holes in the cap were filled one-third with sucrose solution (10%). In each tube, a single leaf was maintained vertically by passing the petiole through the hole so that the petiole remained dipped in solution to remain turgid. A conidial suspension (106 conidia/ml) was inoculated at 2 to 3 ml/leaf with an atomizer. Sterile water was applied to leaves as the control. For each isolate, five leaves were inoculated. Inoculated and control leaves were maintained separately at 25 ± 2°C with a 12-h photoperiod and >85% relative humidity. Typical leaf spot symptoms were observed on inoculated leaves 5 days postinoculation, whereas control leaves remained symptomless. The fungus was reisolated from inoculated leaves and confirmed as C. cassiicola. The internal transcribed spacer region of rDNA was amplified with ITS-1/ITS-4 universal primers and sequenced. Resulting sequences submitted to GenBank (MG976657, MG976661, and MG976664) showed 100% identity with C. cassiicola from avocado (KU593529). Sarbhoy et al. (1971) documented the occurrence of target leaf spot on cotton from southern India; however, this is the first record of C. cassiicola on cotton from central India. Because the disease has the potential to be severe, it could become a significant concern for cotton growers in central India. In the future, management considerations may include early disease forecasting.The author(s) declare no conflict of interest.References:Butler, S., et al. 2016. Plant Dis. 100:535. https://doi.org/10.1094/PDIS-07-15-0785-PDN Link, ISI, Google ScholarConner, K. N., et al. 2013. Plant Dis. 97:1379. https://doi.org/10.1094/PDIS-02-13-0133-PDN Link, ISI, Google ScholarJones, J. P. 1961. Phytopathology 51:305. ISI, Google ScholarSarbhoy, A. K., et al. 1971. Fungi of India. Navyug Traders, New Delhi, India. Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 7 July 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionApple cultivar Joya Cripps Red lesions caused by Colletotrichum fructicola (Nodet et al.). Photo credit: P. Nodet. Symptoms of Lotus powdery mildew caused by Erysiphe takamatsui (Zhou et al.). Photo credit: C. Liang. Symptoms of tar spot (Phyllachora maydis) on maize leaves (Dalla Lana et al.). Photo credit: F. Dalla Lana. Metrics Article History Issue Date: 20 Jun 2019Published: 20 May 2019Accepted: 20 Mar 2019 Page: 1785 Information© 2019 The American Phytopathological SocietyKeywordsfield cropspathogen detectionfungiThe author(s) declare no conflict of interest.Cited byFirst Report of Corynespora cassiicola Causing Target Spot on Cotton (Gossypium hirsutum) in South IndiaMohan Venkata Siva Prasad Bandi, S. L. Bhattiprolu, V. Prasanna Kumari, V. Manoj Kumar, V. Divyamani, A. K. Patibanda, K. Jayalalitha, and D. V. Sai Ram Kumar20 July 2022 | Plant Disease, Vol. 106, No. 8Evaluation of fungicides and bacterial antagonists for management of Corynespora leaf spot on mungbean ( Vigna radiata L. Wilczek)25 January 2022 | Archives of Phytopathology and Plant Protection, Vol. 55, No. 4Cotton Breeding6 May 2022First Report of Leaf Vein Spot Caused by Corynespora cassiicola on Castor Bean in ChinaJ. R. Tang, Y. L. Liu, X. G. Yin, J. N. Lu, and Y. H. Zhou17 September 2020 | Plant Disease, Vol. 104, No. 11Antagonistic potential and phylogeny of culturable endophytic fungi isolated from desi cotton (Gossypium arboreum L.)South African Journal of Botany, Vol. 134