First Report of Dragon Fruit Stem Canker Caused by Lasiodiplodia theobromae in Bangladesh
2019; American Phytopathological Society; Volume: 103; Issue: 10 Linguagem: Inglês
10.1094/pdis-03-19-0619-pdn
ISSN1943-7692
AutoresPreangka Saha Briste, MAH Bhuiyan, Abdul Mannan Akanda, Oliul Hassan, Nur Uddin Mahmud, Md. Abdul Kader, Taehyun Chang, Tofazzal Islam,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 103, No. 10First Report of Dragon Fruit Stem Canker Caused by Lasiodiplodia theobromae in Bangladesh PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Dragon Fruit Stem Canker Caused by Lasiodiplodia theobromae in BangladeshP. S. Briste, M. A. H. B. Bhuiyan, A. M. Akanda, O. Hassan, N. U. Mahmud, M. A. Kader, T. Chang, and M. T. IslamP. S. BristeDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshSearch for more papers by this author, M. A. H. B. BhuiyanDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshSearch for more papers by this author, A. M. AkandaDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshSearch for more papers by this author, O. Hassanhttp://orcid.org/0000-0001-9514-4581Department of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, 37224, Republic of KoreaSearch for more papers by this author, N. U. MahmudInstitute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshSearch for more papers by this author, M. A. KaderDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshSearch for more papers by this author, T. Chang†Corresponding authors: T. Chang; E-mail Address: [email protected] and M. T. Islam; E-mail Address: [email protected]http://orcid.org/0000-0002-0213-7444Department of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, 37224, Republic of KoreaSearch for more papers by this author, and M. T. Islam†Corresponding authors: T. Chang; E-mail Address: [email protected] and M. T. Islam; E-mail Address: [email protected]http://orcid.org/0000-0002-7613-0261Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshSearch for more papers by this authorAffiliationsAuthors and Affiliations P. S. Briste1 M. A. H. B. Bhuiyan1 A. M. Akanda1 O. Hassan2 N. U. Mahmud3 M. A. Kader1 T. Chang2 † M. T. Islam3 † 1Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh 2Department of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, 37224, Republic of Korea 3Institute of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh Published Online:24 Jul 2019https://doi.org/10.1094/PDIS-03-19-0619-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Dragon fruit (Hylocereus polyrhizus) is a newly introduced and promising fruit crop in Bangladesh and has gained considerable interests owing to the fruit’s appealing qualities and characteristics. Recently, stem canker, a new disease of dragon fruit, was observed in 30% of plants at different locations in Bangladesh. Initially, symptoms appeared as small brown lesions, which gradually increased in size. Ultimately, several coalesced lesions gave a stem canker symptom. Isolation of fungi was carried out by the standard isolation procedure (Rodriguez-Galvez et al. 2017). Infected stem samples were surface sterilized in 2% NaOCl for 1 min followed by 70% ethanol for 5 min. Small pieces (2 × 2 mm) from the margin of the necrotic lesion were cut, placed on half-strength potato dextrose agar (PDA) medium, and incubated for 7 days at 25°C. Single-spore isolates (BU-DLa 01 and BU-DLa 02) produced cottony white fluffy colonies on PDA, covering the plate within 3 to 4 days. Mycelial growth was depressed at the center, aerial and fluffy in the rest of the plate. After 5 days, colony color changed from white cottony to dark olive green and black on the reverse side. Black pycnidial bodies were observed on the colony surface after 15 days of incubation at 25°C. Pycnidia were brown to black, subglobose to globose, erumpent, and ostiolated. Immature conidia oozing out from pycnidia were hyaline, thin-walled, globose to subglobose, and single-celled. Mature dark brown conidia were bicellular, thick-walled, and shortly cylindrical (24.98 to 33.32 × 14.48 to 19.94 µm) (average ± SD, 29.39 ± 1.80 × 17.06 ± 1.32) (n = 20). Isolates were morphologically identified as Lasiodiplodia theobromae (Alves et al. 2008). Optimal mycelial growth and sporulation of fungal isolates were obtained at 25 to 30°C and 5.5 to 6.0 pH. At 35°C, reddish pink pigment on the medium was observed. Molecular characterization of isolates was performed using internal transcribed spacer (ITS1, 5.8S rRNA, and ITS2), β-tubulin (TUB), and translation elongation factor 1-alpha (EF-1α) (Carbone and Kohn 1999; Glass and Donaldson 1995; White et al. 1990). ITS sequences of isolates BU-DLa 01 and BU-DLa 02 (GenBank accession nos. LC468780 and LC468781) showed >99% similarity with the sequence from L. theobromae strain H467Z&Z (KU291531). Sequence identity for EF-1α (GenBank accession nos. LC468784 and LC468785) was 99% compared with L. theobromae isolate GZHS-2017-013 (MH454034). TUB sequences (GenBank accession nos. LC468782 and LC468783) were 100% similar with that of L. theobromae isolate DI17-108 (MG523920). Based on the aligned sequences of ITS, TUB, and EF-1α, molecular phylogenetic analysis by the neighbor joining and maximum likelihood method, isolates BU-DLa 01 and BU-DLa 02 were confirmed as L. theobromae (Alves et al. 2008). Fungal isolates were stored on dried filter paper at 4°C at the Department of Plant Pathology at Bangabandhu Sheikh Mujibur Rahman Agricultural University. To confirm pathogenicity, ten 1-month-old seedlings and fruits were also inoculated in vitro using a toothpick technique (Cohen 1994). Three wounds about 5 mm deep were made into the stems with the aid of a sharp sterilized needle and then inoculated using 5-mm mycelial disks from 7-day-old cultures. Wounds were sealed with masking tape to maintain high relative humidity. The control treatment was inoculated with sterile PDA disks. After 2 weeks, all inoculated seedlings produced characteristic symptoms similar to those observed in the field, whereas control seedlings remained healthy. After 3 days of inoculation, fruits also showed rot symptoms, with fungal fructifications on the infected area; control fruits remained healthy. L. theobromae was successfully reisolated on half-strength PDA medium from seedlings, stems, and fruits, fulfilling Koch’s postulates (Cohen 1994). To the best of our knowledge, this is the first report of stem canker of dragon fruit caused by L. theobromae.The author(s) declare no conflict of interest.References:Alves, A., et al. 2008. Fungal Divers. 28:1. ISI, Google ScholarCarbone, I., and Kohn, L. M. 1999. Mycologia 91:553. https://doi.org/10.2307/3761358 Crossref, ISI, Google ScholarCohen, J. 1994. Science 266:1647. Crossref, Google ScholarGlass, N. L., and Donaldson, G. C. 1995. Appl. Environ. Microbiol. 61:1323. Crossref, ISI, Google ScholarRodríguez-Gálvez, E., Guerrero, P., Barradas, C., Crous, P. W., and Alves, A. 2017. Phylogeny and pathogenicity of Lasiodiplodia species associated with dieback of mango in Peru. Fungal Biol. 121:452–465. Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.P. S. Briste and M. A. H. B. Bhuiyan contributed equally to this work.DetailsFiguresLiterature CitedRelated Vol. 103, No. 10 October 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionBell pepper cv. 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Metrics Article History Issue Date: 3 Oct 2019Published: 24 Jul 2019First Look: 23 May 2019Accepted: 21 May 2019 Page: 2686 Information© 2019 The American Phytopathological SocietyKeywordsstem cankerdragon fruitLasiodiplodia theobromaepathogenicityThe author(s) declare no conflict of interest.Cited byEndophytic bacteria isolated from medicinal plants induce plant growth promotion and southern blight disease suppression in tomato6 December 2022 | Journal of Plant Pathology, Vol. 105, No. 1Morphomolecular and cultural characteristics and host range of Lasiodiplodia theobromae causing stem canker disease in dragon fruit31 January 2022 | Journal of Basic Microbiology, Vol. 62, No. 6Diaporthe species causing stem gray blight of red-fleshed dragon fruit (Hylocereus polyrhizus) in Malaysia16 February 2021 | Scientific Reports, Vol. 11, No. 1Bioprospecting for secondary metabolites of family Botryosphaeriaceae from a biotechnological perspective
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