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Virucidal activity of proanthocyanidin against Mayaro virus

2019; Elsevier BV; Volume: 168; Linguagem: Inglês

10.1016/j.antiviral.2019.05.008

1872-9096

Ariane Coelho Ferraz, Thaís de Fátima Silva Moraes, Waleska Stephanie da Cruz Nizer, Michelli dos Santos, Antônio Helvécio Tótola, Jaqueline Maria Siqueira Ferreira, Sidney Augusto Vieira Filho, Vanessa Gonçalves Rodrigues, Lucienir Pains Duarte, Cíntia Lopes de Brito Magalhães, José Carlos de Magalhães,

Research on Leishmaniasis Studies

Mayaro virus (MAYV) is a sublethal arbovirus transmitted by mosquitoes with possible installation of an urban cycle in the Americas. Its infection causes disabling arthralgia, and still, there is no vaccine or treatment to it. We recently investigated nearly 600 compounds by molecular docking and identified epicatechin as a potent antiviral against MAYV. The root extract of Maytenus imbricata showed anti-MAYV activity and two isolated compounds from this plant were also evaluated in vitro. Proanthocyanidin (PAC), a dimer containing epicatechin, showed an effective concentration for 50% of the cells infected by MAYV (EC50) of 37.9 ± 2.4 μM and a selectivity index (SI) above 40. PAC showed significant virucidal activity, inhibiting 100% of the virus proliferation (7 log units), and caused moderate effect during adsorption and virus internalization stage. However, PAC was unable to block the infection when only the cells were pretreated. It was observed a reduction in virus yields when adding PAC at different moments after infection. The set of results indicates that PAC binds to viral and non-cellular elements and may inactivate the MAYV. The inactivation occurs before infection or when the virus reaches the extracellular environment from the 2nd cycle of infection that could block its progression cell-to-cell or to tissues not yet infected.