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Cryopreservation‐induced morphological changes in the sperm of South American silver catfish ( Rhamdia quelen )
2019; Wiley; Linguagem: Inglês
10.1111/jai.13928
ISSN1439-0426
AutoresBruna Bitencourt da Costa, Lis Santos Marques, Paula Graziela Lassen, Rômulo Batista Rodrígues, Danilo Pedro Streit,
Tópico(s)Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
ResumoJournal of Applied IchthyologyVolume 35, Issue 4 p. 987-993 ORIGINAL ARTICLE Cryopreservation-induced morphological changes in the sperm of South American silver catfish (Rhamdia quelen) Bruna B. da Costa, Corresponding Author Bruna B. da Costa bruna.bitencourt@ufrgs.br orcid.org/0000-0002-0836-5018 Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, Brazil Correspondence Bruna B. da Costa, Department of Animal Science, Federal University of Rio Grande do Sul—UFRGS, Bento Gonçalves Avenue, 7712, 91540-000 Porto Alegre, RS, Brazil. Email: bruna.bitencourt@ufrgs.brSearch for more papers by this authorLis S. Marques, Lis S. Marques Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorPaula G. Lassen, Paula G. Lassen Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorRômulo B. Rodrigues, Rômulo B. Rodrigues Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorDanilo P. Streit Jr., Danilo P. Streit Jr. Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this author Bruna B. da Costa, Corresponding Author Bruna B. da Costa bruna.bitencourt@ufrgs.br orcid.org/0000-0002-0836-5018 Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, Brazil Correspondence Bruna B. da Costa, Department of Animal Science, Federal University of Rio Grande do Sul—UFRGS, Bento Gonçalves Avenue, 7712, 91540-000 Porto Alegre, RS, Brazil. Email: bruna.bitencourt@ufrgs.brSearch for more papers by this authorLis S. Marques, Lis S. Marques Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorPaula G. Lassen, Paula G. Lassen Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorRômulo B. Rodrigues, Rômulo B. Rodrigues Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorDanilo P. Streit Jr., Danilo P. Streit Jr. Aquam Research Group, Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this author First published: 31 May 2019 https://doi.org/10.1111/jai.13928Citations: 5 Data Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The cryopreservation protocols are species-specific owing to variable sperm sensitivity towards temperature reduction and contact with cryoprotectant solutions. However, little is known about spermatic pathologies, especially after the cryopreservation process. Thus, the objective was to evaluate the effect of cryopreservation on morphological changes in semen of jundiá (Rhamdia quelen). Sperm pool of five males, with >80% motility, as collected, diluted in a cryoprotectant solution and frozen in liquid nitrogen (−196°C). There was a reduction in the percentage of normal cells and sperm motility, accompanied by an increase in the percentage of sperm abnormalities after cryopreservation of R. quelen spermatozoa, indicating a substantial fragility of the spermatozoa towards the cryopreservation process. The most frequent types of morphological changes in the cryopreserved semen were macrocephaly, folded tail, strongly curled tail and distally curled tail. This is the first study to evaluate the spermatic morphology of R. quelen before and after cryopreservation, paving way for further investigations on morphological alterations and for a new classification of these changes in fish semen due to cryopreservation. CONFLICT OF INTEREST No potential conflict of interest is among the authors. Citing Literature Volume35, Issue4August 2019Pages 987-993 RelatedInformation
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