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Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitória, Espírito Santo, Brazil

2019; BioMed Central; Volume: 12; Issue: 1 Linguagem: Inglês

10.1186/s13071-019-3461-4

1756-3305

Constância Flávia Junqueira Ayres, Duschinka Ribeiro Duarte Guedes, Marcelo Henrique Santos Paiva, Mariana C. Morais-Sobral, Larissa Krokovsky, Laís Ceschini Machado, Maria Alice Varjal de Melo-Santos, Mônica Crespo, Cláudia Maria Fontes de Oliveira, Ricardo Ribeiro, Orlei Amaral Cardoso, Ana Lúcia Barbosa Menezes, Roberto Costa Laperrière-Jr, Carlos Feitosa Luna, André Luiz Sá de Oliveira, Walter Soares Leal, Gabriel Luz Wallau,

Insect symbiosis and bacterial influences

Zika virus (ZIKV) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus Aedes. Here, we detected and isolated ZIKV in samples from Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus, collected during the Zika epidemic in Vitória, southeast Brazil. Using quantitative real-time polymerase chain reaction, ZIKV detection was performed in mosquito samples collected from February to April 2016.Overall, six pools of mosquitoes were positive for ZIKV: four of Cx. quinquefasciatus, one of Ae. aegypti and one of Ae. taeniorhynchus. Their genomes were sequenced.These results support and strengthen the hypothesis that other mosquito species can also be involved in ZIKV transmission.