Produção Nacional
Revisado por pares
DNA methylation screening after roux-en Y gastric bypass reveals the epigenetic signature stems from genes related to the surgery per se
2019; BioMed Central; Volume: 12; Issue: 1 Linguagem: Inglês
10.1186/s12920-019-0522-7
ISSN1755-8794
AutoresCarolina Ferreira Nicoletti, Marcela Augusta Souza Pinhel, Ángel Díaz‐Lagares, Felipe F. Casanueva, María Amalia Jácome, Vitor Caressato Pinhanelli, Bruno Affonso Parenti de Oliveira, Ana B. Crujeiras, Carla Barbosa Nonino,
Tópico(s)Diet and metabolism studies
ResumoObesity has been associated with gene methylation regulation. Recent studies have shown that epigenetic signature plays a role in metabolic homeostasis after Roux-en Y gastric bypass (RYGB). To conduct a genome-wide epigenetic analysis in peripheral blood to investigate whether epigenetic changes following RYGB stem from weight loss or the surgical procedure per se. By means of the Infinium Human Methylation 450 BeadChip array, global methylation was analyzed in blood of 24 severely obese women before and 6 months after RYGB and in 24 normal-weight women (controls). In blood cells, nine DMCpG sites showed low methylation levels before surgery, methylation levels increased after RYGB and neared the levels measured in the controls. Additionally, 44 CpG sites associated with the Wnt and p53 signaling pathways were always differently methylated in the severely obese patients as compared to the controls and were not influenced by RYGB. Finally, 1638 CpG sites related to inflammation, angiogenesis, and apoptosis presented distinct methylation in the post-surgery patients as compared to the controls. Bariatric surgery per se acts on CpGs related to inflammation, angiogenesis, and endothelin-signaling. However, the gene cluster associated with obesity remains unchanged, suggesting that weight loss 6 months after RYGB surgery cannot promote this effect.
Referência(s)