Voltar
Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Diversity of metallo-β-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region

2019; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 114; Linguagem: Inglês

10.1590/0074-02760190020

ISSN

1678-8060

Autores

Danielle Murici Brasiliense, Rodrigo Cayô, Ana Paula Streling, Carolina Silva Nodari, Rafael Ribeiro Barata, Poliana Silva Lemos, Janaína Mota de Vasconcelos Massafra, Yan Corrêa Rodrigues, Igor Magalhães, Ana Cristina Gales, Roberta Sodré,

Tópico(s)

Vibrio bacteria research studies

Resumo

The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens.The present study aimed to verify the occurrence of metallo-β-lactamase genes among distinct Acinetobacter species in a hospital located in the Brazilian Amazon Region.Antimicrobial susceptibility profiles were determined by broth microdilution. The genetic relationships among these isolates were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pyrosequencing reads of plasmids carrying the bla NDM-1 gene were generated using the Ion Torrent™ platform sequencing.A total of six isolates carried bla NDM-1: A. baumannii (n = 2), A. nosocomialis (n = 3), and A. pittii (n = 1); three carried bla IMP-1: A. baumannii, A. nosocomialis, and A. bereziniae. Resistance to colistin was observed for an NDM-1-producing A. nosocomialis isolate. Diverse PFGE patterns and sequence types were found among A. nosocomialis and A. baumannii isolates. The bla NDM-1 sequence was inserted in a Tn125 transposon, while the bla IMP-1 was found as a gene cassette of the class 1 integron In86.To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America.

Referência(s)