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Economic evaluation of the development of a phage therapy product for the control of Salmonella in poultry

2019; American Chemical Society; Volume: 35; Issue: 5 Linguagem: Inglês

10.1002/btpr.2852

8756-7938

Mario A. Torres‐Acosta, Viviana Clavijo, Christopher Vaglio, Andrés Fernando González Barrios, Martha J. Vives, Marco Rito‐Palomares,

Viral gastroenteritis research and epidemiology

Abstract Poultry products are one of the major transmission media of Salmonella enteritidis to humans. A promising alternative to reduce the load of Salmonella in poultry are bacteriophages. Elsewhere, a mixture of six bacteriophages has been used successfully, but large‐scale production would be necessary to supply potential poultry market and costs analyses have not been calculated yet. For this, a powerful tool to predict production costs is bioprocess modeling coupled with economic analyses. This work aims to model the scaled‐up production of a six bacteriophages mixture based on a laboratory/pilot‐scale production using Biosolve Process. For the model construction, a combination of experimental and reported data was applied, in which different production alternatives and the range of 1–100% of the Colombian poultry market (at broiler's farm and slaughterhouse) were analyzed. Results indicate that the best cost‐effective process configuration/scale is to use one bioreactor (156 L) for the six bacteriophages, then a 0.45 μm filtration for removal of biomass, and a 0.22 μm filtration for sterility; this to supply the 35% of the market size for broiler farms (equivalent to 210 million chickens). This configuration gives a production cost per chicken of US$ 0.02. Additionally, a sensitivity analysis and a theoretical contrast for understanding the impact that titer and recovery have on production scale determined that titer affects the most the cost and requires optimization. The present works serves as a first, and required, approach for the development of phage therapy products that are alternatives to present‐day pathogens control strategies.