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Otimização e validação intralaboratorial de método analítico por CLAE/UV para identificação e quantificação de p-fenilenodiamina em tinturas de hena para cabelos e sobrancelhas

2019; FUNDAÇÃO OSWALDO CRUZ; Volume: 7; Issue: 2 Linguagem: Inglês

10.22239/2317-269x.01265

ISSN

2317-269X

Autores

Maria Cristina Santa Bárbara, Helena Miyoco Yano, Fernanda Fernandes Farias, Luiz Gasparin, Edilene Afonso Vieira, Luz Marina Trujillo, Valéria Adriana Pereira Martins, Lígia Luriko Miyamaru, Blanca Elena Ortega Markman,

Tópico(s)

Dye analysis and toxicity

Resumo

Introduction: p-Phenylenediamine (PPD), aromatic sensitizing amine, has been added to henna powder to modify its natural color to black, increasing its fixation time, a practice that is prohibited in eyelash and eyebrows dyes. Objective: The objective of this study was to optimize and validate, at an intra-laboratory level, an analytical method by HPLC/UV for identification and quantification of PPD in hen dyes for hair and eyebrows. Method: In the method, C8 reverse phase column, mobile phase 1% triethanolamine (pH 8.4) andacetonitrile (99: 1, v/v), detection 280nm, injection volume: 10uL, flow 1.0 mL/min, column temperature 32°C, run time 10 min, linearity 5-45 μg/mL (n = 5) with correlation coefficient 0.9982, were used. For Cochran test (homoscedasticity): 0.3350 and Critical (0.3934) with 99% confidence. Limits of detection 1.17 μg/mL and quantification 3.54 μg/mL. The coefficient of variation of repeatability was 0.12% and the intermediate precision by F-test yielded p value of 0.283 with 95% of confidence. Accuracy results comprised acceptance criteria of 90%-107%. Results: Of the 19 analyzed samples, 14 presented PPD content between 1.74 and 3.65% w/w, in disagreement with Legislation. Conclusions: The proposed method can contribute to monitoring of quality and safety of use of these products.

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