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RNA-guided DNA insertion with CRISPR-associated transposases

2019; American Association for the Advancement of Science; Volume: 365; Issue: 6448 Linguagem: Inglês

10.1126/science.aax9181

1095-9203

Jonathan Strecker, Alim Ladha, Zachary Gardner, Jonathan L. Schmid‐Burgk, Kira S. Makarova, Eugene V. Koonin, Feng Zhang,

Pluripotent Stem Cells Research

Beyond adaptive immunity Prokaryotic CRISPR-Cas systems defend bacterial cells from phage and plasmid infection. Strecker et al. characterized a CRISPR-Cas system that functions beyond adaptive immunity (see the Perspective by Hou and Zhang). Type V-K CRISPR-Cas from cyanobacteria was associated with a Tn7-like transposon and a natural nuclease–deficient effector Cas12k. Cas12k directed the insertion of Tn7-like transposons into target sites via RNA-guided Tn7 transposition. This system was reprogrammed to efficiently and specifically insert DNA both in vitro and into the Escherichia coli genome. Science , this issue p. 48 ; see also p. 25