Preparative Liquid Chromatography
1980; Oxford University Press; Volume: 18; Issue: 10 Linguagem: Inglês
10.1093/chromsci/18.10.559
ISSN1945-239X
Autores Tópico(s)Various Chemistry Research Topics
ResumoThere are many forms of preparative liquid chromatography (PLC), each with its own problems and solutions. Five types of PLC are recognised: Type 1 is carried out on analytical HPLC columns. Type 2 is obtained by scaling up HPLC size by a factor of 2 to 10. Type 2 PLC can handle samples in the mg range and can be carried out with analytical HPLC instruments. Type 3 PLC is carried out on long-narrow columns of 1 to 3 m length, consisting of several sections, each of 50 × 1 cm joined together. The columns can be packed by a slurry technique with either 10 μm, 20 μm, or 30 μm material. This type of PLC requires a high volume sampling valve and a high capacity, high pressure pump. Sample size can be up to about 1 g. Large scale or Type 4 PLC (g samples and higher) requires an entirely different solution. One approach is to use 20 or 30 μm material, dry-packed by a “shake and turn” procedure in a short-thick column and run at moderate pressures (2–10 kg/cm2). This type of PLC is not “High Pressure” but can be “High Performance” LC. Other approaches to this type of PLC are currently commercially available and are discussed. Still larger scale Type 5 PLC (kg to ton samples) is an industrial reality. Many aspects of PLC need more research, with detection probably being the least developed area.
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