Highly parallel direct RNA sequencing on an array of nanopores
2018; Nature Portfolio; Volume: 15; Issue: 3 Linguagem: Inglês
10.1038/nmeth.4577
ISSN1548-7105
AutoresDaniel R. Garalde, Elizabeth A. Snell, Daniel Jachimowicz, Botond Sipos, Joseph H Lloyd, Mark Bruce, Nadia Pantic, Tigist Admassu, Phillip James, Anthony Warland, Michael R. Jordan, Jonah Ciccone, Sabrina Serra, Jemma Keenan, Samuel Martin, Luke A. McNeill, E. Jayne Wallace, Lakmal Jayasinghe, Chris Wright, Javier Blasco, Stephen L. Young, Denise Brocklebank, Sissel Juul, James Clarke, Andrew J. Heron, Daniel J. Turner,
Tópico(s)RNA and protein synthesis mechanisms
ResumoSequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.
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