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Colletotrichum brevisporum and C. musicola Causing Leaf Anthracnose of Taro ( Colocasia esculenta ) in Mexico

2019; American Phytopathological Society; Volume: 103; Issue: 11 Linguagem: Italiano

10.1094/pdis-05-19-0967-pdn

1943-7692

Alfonso Vásquez‐López, Rogelio Enrique Palacios-Torres, M. Camacho-Tapía, Carlos Granados-Echegoyen, Nelson Bernardi Lima, Ileana Vera Reyes, Juan Manuel Tovar‐Pedraza, Santos Gerardo Leyva‐Mir,

Plant Pathogens and Resistance

HomePlant DiseaseVol. 103, No. 11Colletotrichum brevisporum and C. musicola Causing Leaf Anthracnose of Taro (Colocasia esculenta) in Mexico PreviousNext DISEASE NOTES OPENOpen Access licenseColletotrichum brevisporum and C. musicola Causing Leaf Anthracnose of Taro (Colocasia esculenta) in MexicoA. Vásquez-López, R. E. Palacios-Torres, M. Camacho-Tapia, C. Granados-Echegoyen, N. B. Lima, I. Vera-Reyes, J. M. Tovar-Pedraza, and S. G. Leyva-MirA. Vásquez-LópezInstituto Politécnico Nacional, CIIDIR, Unidad Oaxaca, Santa Cruz Xoxocotlán, 71230, Oaxaca, MexicoSearch for more papers by this author, R. E. Palacios-TorresUniversidad del Papaloapan, Instituto de Agroingeniería, Loma Bonita, 68400, Oaxaca, MexicoSearch for more papers by this author, M. Camacho-TapiaUniversidad Autónoma Chapingo, Laboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal, Texcoco, 56230, Estado de México, MexicoSearch for more papers by this author, C. Granados-EchegoyenCONACYT–Universidad Autónoma de Campeche, Centro de Estudios de Desarrollo Sustentable y Aprovechamiento de la Vida Silvestre, 24079, Campeche, MexicoSearch for more papers by this author, N. B. Limahttp://orcid.org/0000-0002-6145-5119CONICET–Instituto de Patología Vegetal, CIAP-INTA, X5020ICA, Córdoba, ArgentinaSearch for more papers by this author, I. Vera-ReyesCONACYT–Centro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnologia, Saltillo, 25294, Coahuila, MexicoSearch for more papers by this author, J. M. Tovar-Pedrazahttp://orcid.org/0000-0002-8560-959XCentro de Investigación en Alimentación y Desarrollo, Coordinación Culiacán, Laboratorio de Fitopatología, Culiacán, 80110, Sinaloa, MexicoSearch for more papers by this author, and S. G. Leyva-Mir†Corresponding author: S. G. Leyva-Mir; E-mail Address: lsantos@correo.chapingo.mxhttp://orcid.org/0000-0003-1831-2806Universidad Autónoma Chapingo, Departamento de Parasitología Agrícola, Texcoco, 56230, Estado de México, MexicoSearch for more papers by this authorAffiliationsAuthors and Affiliations A. Vásquez-López1 R. E. Palacios-Torres2 M. Camacho-Tapia3 C. Granados-Echegoyen4 N. B. Lima5 I. Vera-Reyes6 J. M. Tovar-Pedraza7 S. G. Leyva-Mir8 † 1Instituto Politécnico Nacional, CIIDIR, Unidad Oaxaca, Santa Cruz Xoxocotlán, 71230, Oaxaca, Mexico 2Universidad del Papaloapan, Instituto de Agroingeniería, Loma Bonita, 68400, Oaxaca, Mexico 3Universidad Autónoma Chapingo, Laboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal, Texcoco, 56230, Estado de México, Mexico 4CONACYT–Universidad Autónoma de Campeche, Centro de Estudios de Desarrollo Sustentable y Aprovechamiento de la Vida Silvestre, 24079, Campeche, Mexico 5CONICET–Instituto de Patología Vegetal, CIAP-INTA, X5020ICA, Córdoba, Argentina 6CONACYT–Centro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnologia, Saltillo, 25294, Coahuila, Mexico 7Centro de Investigación en Alimentación y Desarrollo, Coordinación Culiacán, Laboratorio de Fitopatología, Culiacán, 80110, Sinaloa, Mexico 8Universidad Autónoma Chapingo, Departamento de Parasitología Agrícola, Texcoco, 56230, Estado de México, Mexico Published Online:28 Aug 2019https://doi.org/10.1094/PDIS-05-19-0967-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Taro (Colocasia esculenta var. antiquorum), family Araceae, is an important tropical crop commonly cultivated throughout the world for its edible corms. During the summer of 2017, severe symptoms of anthracnose were observed on ∼40% of taro plants in a commercial orchard located in San Juan Bautista Tuxtepec, Oaxaca, Mexico. Lesions on taro leaves were irregular, brown, and variable in size. For fungal isolation, small pieces from adjacent tissue to lesions of five symptomatic leaves were surface disinfested by immersion in a 1% sodium hypochlorite solution for 2 min, rinsed in sterile distilled water, and placed in Petri plates containing potato dextrose agar. The plates were incubated at 25°C for 5 days in darkness. Colletotrichum-like colonies were consistently isolated, and 10 monoconidial isolates were obtained. Two isolates were selected as representative for morphological characterization, multilocus phylogenetic analysis, and pathogenicity tests. The isolates were designated as UACH289 and UACH290 and were deposited in the Culture Collection of Phytopathogenic Fungi at the Chapingo Autonomous University. Conidia (n = 100) of isolate UACH289 were cylindrical, hyaline, aseptate, 15.1 to 18.4 × 4.4 to 5.2 μm, with the apex rounded and the base rounded to truncate; appressoria (n = 20) were single, dark brown, and with undulate or lobate margin. Conidia (n = 100) of isolate UACH290 were cylindrical to ellipsoidal, hyaline, aseptate, 12.3 to 17.0 × 3.8 to 5.0 μm; appressoria (n = 20) were irregular, bullet-shaped to elliptical, dark brown, with undulate or lobate margin. For molecular identification, the internal transcribed spacer (ITS) region (White et al. 1990) and fragments of actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin (TUB2) genes were amplified by PCR (Weir et al. 2012) and sequenced. A phylogenetic analysis based on Bayesian inference and including published ITS, ACT, GAPDH, and TUB2 data for Colletotrichum species was performed. After phylogenetic analysis, isolates UACH289 and UACH290 clustered with C. brevisporum (accession nos.: ITS, MK862121; ACT, MK862124; GAPDH, MK862122; and TUB2, MK862123) and C. musicola (accession nos.: ITS, MK882586; ACT, MK882589; GAPDH, MK882587; and TUB2, MK882588), respectively. Pathogenicity of the fungi was verified by spraying of a conidial suspension (1 × 106 spores/ml) on the upper surface of 10 taro leaves. Ten control leaves were sprayed using sterilized water. All plants were kept under greenhouse conditions at 25°C for 10 days. Anthracnose symptoms were observed on all inoculated leaves after 12 days, whereas control leaves remained symptomless. Koch’s postulates were fulfilled when the fungi were reisolated 100% from the diseased leaves. The experiment was performed twice. C. brevisporum has been previously reported as a pathogen on several crops, including Annona muricata, Carica papaya, Sechium edule, Capsicum annuum, Passiflora edulis, Citrus medica, P. edulis, Cucurbita moschata, and C. pepo (Farr and Rossman 2019), whereas C. musicola only has been associated with Musa sp. in Mexico (Damm et al. 2019). To our knowledge, this is the first report of C. brevisporum and C. musicola causing leaf anthracnose of taro in Mexico and worldwide.The author(s) declare no conflict of interest.References:Damm, U., et al. 2019. Stud. Mycol. 92:1. https://doi.org/10.1016/j.simyco.2018.04.001 Crossref, ISI, Google ScholarFarr, D. F., and Rossman, A. Y. 2019. Fungal Databases, Syst. Mycol. Microbiol. Lab., ARS, USDA. Retrieved April 15, 2019, from https://nt.ars-grin.gov/fungaldatabases/. Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 11 November 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionAdvanced symptoms of bacterial blotch disease on mushroom caps (Osdaghi et al.). Photo credit: C. Bull. Powdery mildew caused by Golovinomyces neosalviae on Salvia fruticosa (Soylu et al.). Photo credit: S. Soylu. Metrics Article History Issue Date: 4 Nov 2019Published: 28 Aug 2019First Look: 22 Jun 2019Accepted: 16 Jun 2019 Pages: 2963-2963 Information© 2019 The American Phytopathological SocietyKeywordsfungipathogen diversityphylogenyThe author(s) declare no conflict of interest.Cited byBiocontrol of Colletotrichum brevisporum in soybean using a new genistein-producing Paecilomyces strainBiological Control, Vol. 169Colletotrichum brevisporumCABI Compendium, Vol. CABI CompendiumColletotrichum pereskiae sp. nov. causing anthracnose on Pereskia aculeata in Brazil2 December 2021 | Mycological Progress, Vol. 20, No. 12Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1First Report of Colletotrichum musicola Causing Soybean Anthracnose in BrazilT. R. Boufleur, R. R. L. Castro, F. Rogério, M. Ciampi-Guillardi, R. Baroncelli, and N. S. Massola Júnior17 April 2020 | Plant Disease, Vol. 104, No. 6