Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Designer drugs analysis by LDI(+), MALDI(+) and MALDI(+) imaging coupled to FT-ICR MS

2019; Elsevier BV; Volume: 149; Linguagem: Inglês

10.1016/j.microc.2019.104002

1095-9149

Camila Medeiros de Almeida, Fernanda E. Pinto, Nayara Araújo dos Santos, Lindamara M. Souza, Bianca B. Merlo, Christopher J. Thompson, Wanderson Romão,

Psychedelics and Drug Studies

Abstract The phenethylamines derivatives, the NBOMes, N-bomb or Smiles, are potent hallucinogens, which are often sold as blotter paper. In this study, the matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) and MALDI mass spectrometry imaging (MALDI-MSI) were coupled to a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS), a high-resolution equipment with a mass accuracy lower than 4 ppm, and used to analyze seven blotter papers of NBOMes containing 25I-NBOH (2-({[2-(4-iodo-2,5-dimethoxyphenyl)ethyl]amino}methyl) phenol, m/z 414) and 25I-NBOMe ((2-(4-iodo-2,5-dimethoxyphenyl)-N-[(2-methoxyphenyl) methyl]ethanamine), m/z 428). An optimization study was performed to evaluate the matrix application (pipette versus sprayer assisted by an ESI probe); the matrix effect in the process of desorption and ionization (α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), 7,7,8,8-tetracyanoquinodimethane (TCNQ) and sinapinic acid (SA)); and their concentration (from 5 to 25 mg mL−1). After, a study of the spatial distribution of the NBOMes derivatives detected in the front and back of the blotter paper surface was performed. MALDI-MS and MALDI-MSI results showed that the matrix sprayer was the best method for matrix application, which allowed a homegeneous detection of the compound when compared to the pipette application. The CHCA matrix, in a concentration higher than 15 mg mL−1, displayed a higher ionization efficiency of the 25I-NBOMe compound, commonly detected in blotter paper, where an intense distribution was observed in relation to others matrices. The distribution of the active ingredient studied in the front and back between the six blotter papers analyzed showed that the three of them had a higher concentration of the active ingredient in only one of the sides (two in the back and one in the front), while the others presented a similar distribution in both sides. The ability of MALDI(+)MS technique in 25I-NBOMe quantification (from 0.0125 to 0.1 mg mL−1) was also evaluated, where a better sensitivity and linearity (R2 0.9852) of the method was observed when high matrix concentrations (15–20 mg mL−1) were used.