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BIBTEX RIS

Anti‐inflammatory activity of β ‐caryophyllene combined with docosahexaenoic acid in a model of sepsis induced by Staphylococcus aureus in mice

2019; Wiley; Volume: 99; Issue: 13 Linguagem: Inglês

10.1002/jsfa.9861

ISSN

1097-0010

Autores

Laís Ferraz Brito, Hellen Braga Martins Oliveira, Nathan das Neves Selis, Clarissa Leal Silva e Souza, Manoel Neres Santos Júnior, Érika Pereira de Souza, Lucas Santana Coelho da Silva, Flávia de Souza Nascimento, Aline Teixeira Amorim, Guilherme Barreto Campos, Hellen Braga Martins Oliveira, Regiane Yatsuda, Jorge Timenetsky, Lucas Miranda Marques,

Tópico(s)

Biochemical Analysis and Sensing Techniques

Resumo

BACKGROUND Sepsis is a set of serious organic manifestations caused by an infection, whose progression culminates in exacerbated inflammation and oxidative stress, poor prognosis, and high hospital costs. Antioxidants used against sepsis have been evaluated, including essential oils such as β ‐caryophyllene (BCP), and polyunsaturated fatty acids, such as docosahexaenoic acid (DHA). The aim of this study was to evaluate the anti‐inflammatory activity of the association of these two compounds. RESULTS Treatment with BCP‐DHA, at a dose of 200 μL/animal, significantly inhibited the migration of neutrophils in a Cg‐induced peritonitis model. After Staphylococcus aureus infection, in the groups treated with BCP‐DHA there was a significant decrease in the total and differential count of leukocytes, increased expression of cytokines TNF‐ α and IFN‐ γ in treated groups, an increase of IL‐4 and IL‐5 in B/D and B/D + SA groups, and an augmentation of IL‐6 and IL‐12 groups in B/D + SA groups. Histological and bacterial analysis revealed lower neutrophil migration and lower bacterial load in the infected and treated groups. CONCLUSION In general, the BCP‐DHA association presented anti‐inflammatory activity against two different models of acute inflammation and infection, showing promising potential as a therapeutic adjuvant in sepsis. © 2019 Society of Chemical Industry

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