Novel steroids from Trichilia hirta as identified by nanoprobe INADEQUATE 2D-NMR spectroscopy
1996; Elsevier BV; Volume: 37; Issue: 44 Linguagem: Inglês
10.1016/0040-4039(96)01803-5
ISSN1873-3581
AutoresDenise C. Chauret, Tony Durst, John T. Arnason, Pablo Sánchez-Vindas, L. San Román, Luis Poveda, Paul A. Keifer,
Tópico(s)Plant Diversity and Evolution
ResumoTwo novel steroids, 3-hydroxypregnane-2,16-dione [1] and 2-hydroxyandrosta-1,4-diene-3,16 dione [2], were isolated from ethanolic extracts of the wood and bark of Trichilia hirta. The structure of 1 was rigorously determined from 2D-INADEQUATE NMR data that was: 1) acquired with a new higher-sensitivity 13C probe called a Nano·nmr probe, and; 2) processed with a new NMR-analysis program called FRED, which automatically generated the complete carbon structure (as shown). In this first known application of both a Nanoprobe and FRED software to an unknown compound, the complete carbon skeleton was easily determined using only 11 mg (30 μmoles) of sample.
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