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Biocompatible and Antimicrobial Electrospun Membranes Based on Nanocomposites of Chitosan/Poly (Vinyl Alcohol)/Graphene Oxide

2019; Multidisciplinary Digital Publishing Institute; Volume: 20; Issue: 12 Linguagem: Inglês

10.3390/ijms20122987

1661-6596

Julián Andrés Tamayo Marín, Sebastián Ruiz Londoño, Johannes Delgado‐Ospina, Diana Paola Navia Porras, Mayra Eliana Valencia Zapata, José Hermínsul Mina Hernández, Carlos Humberto Valencia-Llano, Carlos David Grande‐Tovar,

Bone Tissue Engineering Materials

Tissue engineering is gaining attention rapidly to replace and repair defective tissues in the human body after illnesses and accidents in different organs. Electrospun nanofiber scaffolds have emerged as a potential alternative for cell regeneration and organ replacement. In this paper, porous membranes, based on nanofibrous chitosan (CS), polyvinyl alcohol (PVA), and graphene oxide (GO), were obtained via electrospinning methodology. Three different formulations were obtained varying GO content, being characterized by Fourier Transform Infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). In vitro tests were carried out, consisting of hydrolytic degradation inside simulated biological fluid (SBF), and in vivo tests were carried out, where the material was implanted in Wistar rats' subcutaneous tissue to determine its biocompatibility. The antibacterial activity was tested against Gram-positive bacteria Bacilluscereus and Staphylococcusaureus, and against Gram-negative Salmonellaenterica and Escherichiacoli, by contact of the electrospun nanofiber scaffolds above inoculum bacterial in Müeller Hinton agar with good inhibition only for scaffolds with the higher GO content (1.0%). The results confirmed good biocompatibility of the nanofibrous scaffolds after in vivo tests in Wistar rats, which evidences its high potential in applications of tissue regeneration.