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Differential analysis of RNA-Seq incorporating quantification uncertainty

2016; Cold Spring Harbor Laboratory; Linguagem: Inglês

10.1101/058164

Harold Pimentel, Bray Nicolas L., Suzette Puente, Páll Melsted, Lior Pachter,

RNA and protein synthesis mechanisms

We describe a novel method for the differential analysis of RNA-Seq data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. The method is implemented in an interactive shiny app called sleuth that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of RNA-Seq experiments.