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Single-cell transcriptome conservation in cryopreserved cells and tissues

2017; BioMed Central; Volume: 18; Issue: 1 Linguagem: Inglês

10.1186/s13059-017-1171-9

1474-760X

Amy Guillaumet-Adkins, Gustavo Rodríguez-Esteban, Elisabetta Mereu, María Méndez-Lago, Diego Adhemar Jaitin, Alberto Villanueva, August Vidal, Alex Martínez‐Martí, Enriqueta Felip, Ana Vivancos, Hadas Keren‐Shaul, Simon Heath, Marta Gut, Ido Amit, Marta Gut, Holger Heyn,

RNA Research and Splicing

A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.