Activity-Based Probe for Screening of High-Colibactin Producers from Clinical Samples
2019; American Chemical Society; Volume: 21; Issue: 12 Linguagem: Inglês
10.1021/acs.orglett.9b01345
ISSN1523-7060
AutoresYuichiro Hirayama, Yuta Tsunematsu, Yuko Yoshikawa, Ryota Tamafune, Nobuo Matsuzaki, Yuji Iwashita, Ippei Ohnishi, Fumihiko Tanioka, Michio Sato, Noriyuki Miyoshi, Michihiro Mutoh, Hideki Ishikawa, Haruhiko Sugimura, Keiji Wakabayashi, Kenji Watanabe,
Tópico(s)Genomics and Phylogenetic Studies
ResumoWhile high-colibactin-producing Escherichia coli is thought to be associated with colorectal oncogenesis, this study is complicated part due to an inability to isolate colibactin adequately. Here, we created fluorescent probes activated by ClbP, the colibactin-maturing peptidase, to identify high-colibactin-producing strains. Our probe served as a valuable clinical diagnostic tool that allowed simple high-throughput diagnostic screening of clinical samples. Furthermore, the probe also allowed identification of high-colibactin producers that would help advance our understanding of colibactin biosynthesis.
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