Portal de Periódicos da CAPES

First Report of Black Root Rot Disease on Morinda officinalis Caused by Lasiodiplodia pseudotheobromae in China

2019; American Phytopathological Society; Volume: 103; Issue: 10 Linguagem: Inglês

10.1094/pdis-04-19-0857-pdn

1943-7692

Zhangyong Dong, Yongxin Shu, Mei Luo, Weili Zhang, Xinyu Chen, Yongxin Xiao, Meimei Xiang,

Mycorrhizal Fungi and Plant Interactions

HomePlant DiseaseVol. 103, No. 10First Report of Black Root Rot Disease on Morinda officinalis Caused by Lasiodiplodia pseudotheobromae in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Black Root Rot Disease on Morinda officinalis Caused by Lasiodiplodia pseudotheobromae in ChinaZhangyong Dong, Yongxin Shu, Mei Luo, Weili Zhang, Xinyu Chen, Yongxin Xiao, and Meimei XiangZhangyong Dong†Corresponding authors: Z. Dong; E-mail Address: dongzhangyong@hotmail.com and M. Xiang; E-mail Address: mm_xiang@163.comhttp://orcid.org/0000-0001-7524-0226Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China, Yongxin ShuZhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China, Mei Luohttp://orcid.org/0000-0002-1950-4204Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China, Weili ZhangZhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China, Xinyu ChenZhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China, Yongxin XiaoZhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China, and Meimei Xiang†Corresponding authors: Z. Dong; E-mail Address: dongzhangyong@hotmail.com and M. Xiang; E-mail Address: mm_xiang@163.comZhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of ChinaAffiliationsAuthors and Affiliations Zhangyong Dong † Yongxin Shu Mei Luo Weili Zhang Xinyu Chen Yongxin Xiao Meimei Xiang † Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China Published Online:7 Aug 2019https://doi.org/10.1094/PDIS-04-19-0857-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Morinda officinalis is a wildly cultivated medicinal plant in Zhaoqing, Guangdong Province of China. The annual production of M. officinalis in Deqin county of Zhaoqing accounts for 90% of China's total production. In 2018, a disease on M. officinalis caused significant economic losses by affecting medicinal plant quality. Symptomatic plants exhibited blackened necrotic discoloration of roots. A diseased plant was collected in June 2018 from Zhaoqing, Guangdong, surface sterilized in 75% ethanol for 1 min and then in 2% NaClO for 3 min, and rinsed three times in sterile distilled water; internal necrotic tissue was then transferred to potato dextrose agar (PDA) and incubated at 28°C for 3 days. The fungal colonies were initially white and then became green to dark green with sporulation. Some small black pellets were found on the edge of the colony cultured on PDA medium for 25 days. The morphology of black fungal pellets was examined by light microscopy. The conidia were ellipsoidal, initially hyaline, unicellular, becoming dark brown, and developing a thick wall and a central septum. Conidia measured 16.01 to 21.74 μm long and 8.83 to 11.43 μm wide (n = 60). The conidial morphology matched that of Lasiodiplodia, a member of the Botryosphaeriaceae family (Alves et al. 2008). Moreover, the internal transcribed spacer region of the ribosomal RNA was amplified by using primers ITS1 and ITS4 (White et al. 1990) and sequenced (GenBank accession no. MK090538). The 542-bp sequence was compared with the GenBank database using nucleotide BLAST, and the isolate sequence was 100% similar to the sequence of Lasiodiplodia pseudotheobromae (GenBank accession no. MH663527). The β-tubulin gene region of the ribosomal DNA was amplified by using primers T1 (O'Donnell and Cigelnik 1997) and CYLTUB1R (Crous et al. 2004) and sequenced (GenBank accession no. MK328528). The 459-bp sequence was compared with the GenBank database using nucleotide BLAST, and the isolate sequence was 100% similar to the sequence of L. pseudotheobromae (GenBank accession no. KY583260.1). On the basis of morphological characteristics and nucleotide homology, the isolate was identified as L. pseudotheobromae, a member of the Botryosphaeriaceae family. To satisfy Koch's postulates, both roots and leaves of M. officinalis were inoculated by placing a mycelium plug from the growing margin of 7-day-old colonies upside down directly into a fresh wound. After 3 days, the root xylem of inoculated plants turned brown and gradually became dark, similar to symptoms observed in the field. The leaves also turned brown and gradually became dark brown after 4 days. The disease spots were round or nearly circular. No symptoms were observed on the control plants. The pathogen was reisolated from root lesions, and its identity was confirmed by morphological characteristics. To our knowledge, this is first report of L. pseudotheobromae causing black root rot of M. officinalis in China.The author(s) declare no conflict of interest.References:Alves, A., et al. 2008. Fungal Divers. 28:1. ISI, Google ScholarCrous, P. W., et al. 2004. Stud. Mycol. 50:415. Google ScholarO'Donnell, K., and Cigelnik, E. 1997. Mol. Phylogenet. Evol. 7:103. https://doi.org/10.1006/mpev.1996.0376 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.Z. Dong and Y. Shu contributed equally to this work.Funding: This work was supported by Guangdong Technological Innovation Strategy of Special Funds (Key Areas of Research and Development Program), grant no. 2018B020205003.DetailsFiguresLiterature CitedRelated Vol. 103, No. 10 October 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionBell pepper cv. Bomby infected with zucchini yellow mosaic virus along with cucumber mosaic virus, pepper mild mottle virus, and tobacco mosaic virus (Verma et al.). Photo credit: S. Tripathi. Severe stunting, leaf chlorosis, and horizontal head growth of sunflower infected with Plasmopara halstedii, causal agent of downy mildew (Humann et al.). Photo credit: S. Markell. Metrics Article History Issue Date: 3 Oct 2019Published: 7 Aug 2019First Look: 17 Jun 2019Accepted: 13 Jun 2019 Page: 2693 Information© 2019 The American Phytopathological SocietyFundingGuangdong Technological Innovation Strategy of Special Funds (Key Areas of Research and Development Program)Grant/Award Number: 2018B020205003KeywordsMorinda officinalisblack root rot diseaseLasiodiplodia pseudotheobromaeThe author(s) declare no conflict of interest.Cited byWhole-Genome Resource of Lasiodiplodia pseudotheobromae BaA, the Causative Agent of Black Root Rot Morinda officinalisXiaoyi Li, Mei Luo, Handa Song, and Zhangyong Dong31 December 2022 | Plant Disease, Vol. 107, No. 2Endophytic Diaporthe Associated with Morinda officinalis in China29 July 2022 | Journal of Fungi, Vol. 8, No. 8Lasiodiplodia pseudotheobromaeCABI Compendium, Vol. CABI CompendiumBioprospecting for secondary metabolites of family Botryosphaeriaceae from a biotechnological perspective