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Evaluation of growth performance and lipid metabolism in zebrafish fed fructooligosaccharide using RNA sequencing

2019; Wiley; Volume: 25; Issue: 6 Linguagem: Inglês

10.1111/anu.12934

1365-2095

Chunnuan Zhang, Junhui Wang, Maoxian Huang, Min Liu, Jiliang Zhang,

Reproductive biology and impacts on aquatic species

Aquaculture NutritionVolume 25, Issue 6 p. 1194-1206 ORIGINAL ARTICLE Evaluation of growth performance and lipid metabolism in zebrafish fed fructooligosaccharide using RNA sequencing Chunnuan Zhang, Corresponding Author Chunnuan Zhang zhangchunnuan12@163.com orcid.org/0000-0003-4376-0969 Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China Correspondence Chunnuan Zhang and Jiliang Zhang, College of Animal Science and Technology, Henan University of Science and Technology, 263 Kaiyuan Road, Luoyang, Henan, 471023, China. Emails: zhangchunnuan12@163.com (C.-n. Zhang); jlzhang@haust.edu.cn (J.-l. Zhang)Search for more papers by this authorJunhui Wang, Junhui Wang Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaSearch for more papers by this authorMaoxian Huang, Maoxian Huang Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China College of Ocean Sciences, Hainan University, Haikou, ChinaSearch for more papers by this authorMin Liu, Min Liu Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaSearch for more papers by this authorJiliang Zhang, Corresponding Author Jiliang Zhang jlzhang@haust.edu.cn Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China Correspondence Chunnuan Zhang and Jiliang Zhang, College of Animal Science and Technology, Henan University of Science and Technology, 263 Kaiyuan Road, Luoyang, Henan, 471023, China. Emails: zhangchunnuan12@163.com (C.-n. Zhang); jlzhang@haust.edu.cn (J.-l. Zhang)Search for more papers by this author Chunnuan Zhang, Corresponding Author Chunnuan Zhang zhangchunnuan12@163.com orcid.org/0000-0003-4376-0969 Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China Correspondence Chunnuan Zhang and Jiliang Zhang, College of Animal Science and Technology, Henan University of Science and Technology, 263 Kaiyuan Road, Luoyang, Henan, 471023, China. Emails: zhangchunnuan12@163.com (C.-n. Zhang); jlzhang@haust.edu.cn (J.-l. Zhang)Search for more papers by this authorJunhui Wang, Junhui Wang Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaSearch for more papers by this authorMaoxian Huang, Maoxian Huang Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China College of Ocean Sciences, Hainan University, Haikou, ChinaSearch for more papers by this authorMin Liu, Min Liu Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaSearch for more papers by this authorJiliang Zhang, Corresponding Author Jiliang Zhang jlzhang@haust.edu.cn Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China Correspondence Chunnuan Zhang and Jiliang Zhang, College of Animal Science and Technology, Henan University of Science and Technology, 263 Kaiyuan Road, Luoyang, Henan, 471023, China. Emails: zhangchunnuan12@163.com (C.-n. Zhang); jlzhang@haust.edu.cn (J.-l. Zhang)Search for more papers by this author First published: 03 July 2019 https://doi.org/10.1111/anu.12934Citations: 4Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract This study evaluated the effects of fructooligosaccharide (FOS) on growth performance and lipid metabolism in zebrafish (Danio rerio) by RNA sequencing. A total of 240 healthy zebrafish were randomly distributed into two groups. The control group was fed a basal diet, and the treatment group was fed a basal diet supplemented with 0.4% FOS. The results showed that there was no significant difference in growth performance (p > 0.05). The lipid content, total cholesterol, triglyceride, free fatty acid and low-density lipoprotein were significantly lower (p < 0.05) in the liver of fish fed the 0.4% FOS diet than those of the fish fed the control diet, while the fish fed the 0.4% FOS diet had significantly higher (p < 0.05) high-density lipoproteins than those of the control fish. Malic enzyme and fatty acid synthetase activities were significantly reduced by adding FOS. KEGG pathway analysis showed that the two pathways of steroid hormone biosynthesis and steroid biosynthesis were significantly enriched (p < 0.05). The profile of genes in these two pathways was affected by FOS in zebrafish. The results of the two pathways suggested new mechanisms underlying the lipid metabolism mechanism of FOS. Citing Literature Supporting Information Filename Description anu12934-sup-0001-Additionalfile.xlsMS Excel, 46.9 KB Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume25, Issue6December 2019Pages 1194-1206 RelatedInformation