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BIBTEX RIS

Exogenous expression of caveolin‐1 is sufficient for hepatic stellate cell activation

2019; Wiley; Volume: 120; Issue: 11 Linguagem: Inglês

10.1002/jcb.29226

1097-4644

Mariana Ilha, Ketlen da Silveira Moraes, Francieli Rohden, Léo Anderson Meira Martins, Radovan Borojević, Guido Lenz, Florencia María Barbé‐Tuana, Fátima Theresinha Costa Rodrigues Guma,

Liver Disease Diagnosis and Treatment

Abstract Caveolin‐1 (Cav‐1) expression is increased in hepatic stellate cells (HSC) upon liver cirrhosis and it functions as an integral membrane protein of lipid rafts and caveolae that regulates and integrates multiple signals as a platform. This study aimed to evaluate the role of Cav‐1 in HSC. Thus, the effects of exogenous expression of Cav‐1 in GRX cells, a model of activated HSC, were determined. Here, we demonstrated through evaluating well‐known HSC activation markers – such as α‐smooth muscle actin, collagen I, and glial fibrillary acidic protein – that up regulation of Cav‐1 induced GRX to a more activated phenotype. GRX EGFP‐Cav1 presented an increased migration, an altered adhesion pattern, a reorganization f‐actin cytoskeleton, an arrested cell cycle, a modified cellular ultrastructure, and a raised endocytic flux. Based on this, GRX EGFP‐Cav1 represents a new cellular model that can be an important tool for understanding of events related to HSC activation. Furthermore, our results reinforce the role of Cav‐1 as a molecular marker of HSC activation.