Produção Nacional
Revisado por pares
Environmental Assessment and Evaluation of Oxidative Stress and Genotoxicity Biomarkers Related to Chronic Occupational Exposure to Benzene
2019; Multidisciplinary Digital Publishing Institute; Volume: 16; Issue: 12 Linguagem: Inglês
10.3390/ijerph16122240
ISSN1661-7827
AutoresIsabele Campos Costa‐Amaral, Leandro Vargas Barreto de Carvalho, Marcus Vinícius Corrêa dos Santos, Daniel Valente, Angélica Cardoso Pereira, Victor Oliva Figueiredo, Juliana Mendonça de Souza, Vinicio Soares de Castro, Maria de Fátima Trancoso, Antônio Sérgio Almeida Fonseca, Vanessa G. Milagres, Michele P. R. Mendes, Maria José Nunes de Paiva, Leiliane Coelho André, Renato Marçullo Borges, Marco Antônio Carneiro Menezes, Sérgio Rabello Alves, Eline Simões Gonçalves, Herbert Ary Sisenando, Jamila Alessandra Perini, M. Stuck Oliveira, Maria Juliana Moura-Corrêa, Liliane Reis Teixeira, Andrew Collins, Rita de Cássia O. C. Mattos, Paula de Novaes Sarcinelli, Ariane Leites Larentis,
Tópico(s)Indoor Air Quality and Microbial Exposure
ResumoEnvironmental and occupational exposure to benzene from fuels is a major cause for concern for national and international authorities, as benzene is a known carcinogen in humans and there is no safe limit for exposure to carcinogens. The objective of this study was to evaluate the genotoxic effects of chronic occupational exposure to benzene among two groups of workers: filling station workers (Group I) and security guards working at vehicles entrances (Group II), both on the same busy highway in Rio de Janeiro, Brazil. Sociodemographic data on the workers were evaluated; the concentration of benzene/toluene (B/T) in atmospheric air and individual trans,trans-muconic acid (ttMA) and S-phenylmercapturic acid (S-PMA) were measured; oxidative stress was analyzed by catalase (CAT), glutathione S-transferase (GST), superoxide dismutase (SOD), thiol groups (THIOL) and malondialdehyde (MDA); genotoxicity was measured by metaphases with chromosomal abnormalities (MCA) and nuclear abnormalities, comet assay using the enzyme formamidopyrimidine DNA glycosylase (C-FPG), and methylation of repetitive element LINE-1, CDKN2B and KLF6 genes. Eighty-six workers participated: 51 from Group I and 35 from Group II. The B/T ratio was similar for both groups, but Group I had greater oscillation of benzene concentrations because of their work activities. No differences in ttMA and S-PMA, and no clinical changes were found between both groups, but linearity was observed between leukocyte count and ttMA; and 15% of workers had leukocyte counts less than 4.5 × 109 cells L−1, demanding close worker’s attention. No differences were observed between the two groups for THIOL, MDA, MCA, or nuclear abnormalities. A multiple linear relationship was obtained for the biomarkers MCA and C-FPG. A significant correlation was found between length of time in current job and the biomarkers C-FPG, MCA, GST, and MDA. Although both populations had chronic exposure to benzene, the filling station workers were exposed to higher concentrations of benzene during their work activities, indicating an increased risk of DNA damage.
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