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USO DE LIPOPROTEÍNAS DE BAIXA DENSIDADE E DE GEMA DE OVO NA PRESERVAÇÃO DO SÊMEN ASININO RESFRIADO A 6o C

2012; Sociedade de Medicina Veterinária do Estado do Rio de Janeiro; Volume: 34; Issue: 4 Linguagem: Inglês

2527-2179

Maria Isabel Vaz de Melo, Yolanda de Fátima Resende, Elton Pavão Rezende, Paola Pereira das Neves Snoeck, Daniel de Moura Lima Gomes, Tharley Teixeira Cardoso Carvalho, Alan Vinícius Duarte Silva, Mariana Machado‐Neves, Danilo Gonçalves Bastos, M. Henry,

Sperm and Testicular Function

ABSTRACT. de Melo M.I.V., Resende Y. de F., Rezende E.P., Snoeck P.P. das N., Gomes D. de M.L., Carvalho Th.T.C., Silva A.V.D., Neves M.M., Bastos D.G. & Henry M.R.J.M.[Low density lipoprotein and egg yolk to preserve cooled donkey semen]. Uso de lipoproteinas de baixa densidade e de gema de ovo na preservacao do semen asinino resfriado a 6o C. Revista Brasileira de Medicina Veterinaria, 34(4):288-294, 2012. Departamento de Medicina Veterinaria, Pontificia Universidade Catolica de Minas Gerais, Unidade Betim, Rua do Rosario, 1081, Bairro Angola, Betim, MG 32604- 115, Brasil, E-mail: bel.melo@terra.com.br The aim of this study was to evaluate the characteristics of cooled asinine sperm. Six Pega donkeys were used. Semen was diluted 1:3 in extenders: D1 - Botusemen ®; D2 - Botusemen ® + 10% egg yolk; D3 - Botusemen ® + 10% egg yolk, centrifuged at 800 X g for 15’; D4 - Botusemen ® + 6.8% low density lipoprotein of egg yolk. The diluted semen was submitted to the cooling rate of 0.65° C / minute to achieve 5o C. It was transported and kept in a trade refrigerator at 6° C for three days. It was used the computerized system sperm analysis (CASA) to assess the characteristics of sperm every 24 hours of the cooling period. D3 (Botusemen + egg yolk - centrifuged) was superior compared to other extenders studied the preservation of sperm characteristics, increasing storage time, mainly distinguished themselves in the percentage of motile sperm, rapidly speed, rectilinear and curvilinear velocity of displacement.