First Report of Dickeya fangzhongdai Causing Soft Rot of Phalaenopsis aphrodite in China
2019; American Phytopathological Society; Volume: 103; Issue: 10 Linguagem: Inglês
10.1094/pdis-01-19-0234-pdn
ISSN1943-7692
AutoresYanqiong Shen, Weixing Lv, Yunpeng Du, Y. X. Zhang, Hanpeng Li,
Tópico(s)Irrigation Practices and Water Management
ResumoHomePlant DiseaseVol. 103, No. 10First Report of Dickeya fangzhongdai Causing Soft Rot of Phalaenopsis aphrodite in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Dickeya fangzhongdai Causing Soft Rot of Phalaenopsis aphrodite in ChinaY. Shen, W. G. Lv, Y. H. Du, Y. X. Zhang, and H. P. LiY. Shen†Corresponding author: Y. Shen; E-mail Address: 763399029@qq.comhttp://orcid.org/0000-0002-2600-3854Shunde Office of Foshan Customs, 528303, Foshan, China, W. G. LvShunde Office of Foshan Customs, 528303, Foshan, China, Y. H. DuShunde Office of Foshan Customs, 528303, Foshan, China, Y. X. ZhangShunde Office of Foshan Customs, 528303, Foshan, China, and H. P. LiShunde Office of Foshan Customs, 528303, Foshan, ChinaAffiliationsAuthors and Affiliations Y. Shen † W. G. Lv Y. H. Du Y. X. Zhang H. P. Li Shunde Office of Foshan Customs, 528303, Foshan, China Published Online:19 Aug 2019https://doi.org/10.1094/PDIS-01-19-0234-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Phalaenopsis aphrodite is very popular in China. In the summer of 2017, a soft rot of P. aphrodite was found at the base of Phalaenopsis plants in Foshan city, Guangdong Province, with the incidence of about 10%. The infected leaves displayed water-soaked small round spots initially, and the spots expanded quickly with the increase of humidity and temperature, becoming translucent when facing a light source. Several days later, the inclusions exuded as the lesions decayed. The bacteria further infected the leaf sheath, resulting in the discoloring and perishing of leaf sheaths, falling off of leaves, and even the death of the whole plant. Finally, the leaves became as dry as paper. Five leaves with typical symptoms were randomly collected; after washing with 75% ethanol for 30 s, with 0.1% HgCl2 for 2 min, and with sterile water three times, small pieces of tissues (5 × 5 mm) were removed from lesion borders, plated on LB solid medium, and incubated at 37°C for 24 to 48 h. Thereafter, 10 representative isolates were chosen for further identification. Generally, these strains were gram negative, cultivated at 37°C, and utilized D-arabinose, melibiose, raffinose, and mannitol but not 5-keto-D-gluconate, β-gentiobiose, or casein. A GEN III MicroPlate system (Biolog) was used for examination, and the 10 isolates were identified as Dickeya chrysanthemi (SIM 0.856). PCR was used to amplify the 16S rDNA gene with primers 27f and 1492r (Tillett et al. 2000), recA (Waleron et al. 2002), and dnaX (Sławiak et al. 2009). BLASTn was carried out online, and the underlying phylogeny trees were obtained based on these gene sequences using MEGA 5.05 through 100% bootstrap values. The results showed that representative tested strain FSPAD1, whose sequences were deposited in GenBank under accession numbers MK394174 (16S rDNA), MK405705 (recA), and MK405706 (dnaX), had the highest homology with D. fangzhongdai for 16S rDNA(JN940859), recA (KT992693), and dnaX (KT992713), with the identities of 100, 97, and 97%, respectively. Therefore, this isolate was identified as D. fangzhongdai strain FSPAD1. Pathogenicity tests were performed on healthy tender leaves of P. aphrodite. Sterile filter paper (1 cm in diameter) was used to absorb sterile water and the bacterial suspension (1 × 108 CFU/ml). Then, the paper with sterile water (control) or the bacterial suspension were attached to tender leaves with three acupuncture points in each leaf. All tested plants were incubated at 28°C and kept moist with plastic bags. After 2 days, soft rot symptoms were observed in the pathogen-inoculated leaves, whereas sterile water (control) inoculated leaves exhibited no symptoms. The pathogenicity test was conducted three times. Koch's postulates were achieved by reisolating D. fangzhongdai from the inoculated leaves. Meanwhile, 16S rDNA sequence alignment confirmed that the new reisolation was consistent with that of the inoculated plant. This pathogenic bacterium has been reported on Pyrus pyrifolia in China (Tian et al. 2016). So far, this is the first report of D. fangzhongdai causing soft rot disease on P. aphrodite.The author(s) declare no conflict of interest.References:Sławiak, M., et al., 2009. Eur. J. Plant Pathol. 125:245. https://doi.org/10.1007/s10658-009-9479-2 Google ScholarTian, Y., et al. 2016. Int. J. Syst. Evol. Micr. 66:2831. https://doi.org/10.1099/ijsem.0.001060 Google ScholarTillett D., et al., 2000. J. Phycol. 36:251. https://doi.org/10.1046/j.1529-8817.2000.99079.x Google ScholarWaleron, M., et al. 2002. Plant Prot. Sci. 38:288. https://doi.org/10.1099/00221287-148-2-583 Google ScholarThe author(s) declare no conflict of interest.Funding: The authors are grateful to Funding Project for Science and Technology Planning Project under the Jurisdiction of Guangdong Entry-Exit Inspection and Quarantine Bureau (2018GDK64) for the financial support.DetailsFiguresLiterature CitedRelated Vol. 103, No. 10 October 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionBell pepper cv. Bomby infected with zucchini yellow mosaic virus along with cucumber mosaic virus, pepper mild mottle virus, and tobacco mosaic virus (Verma et al.). Photo credit: S. Tripathi. Severe stunting, leaf chlorosis, and horizontal head growth of sunflower infected with Plasmopara halstedii, causal agent of downy mildew (Humann et al.). Photo credit: S. Markell. Metrics Article History Issue Date: 3 Oct 2019Published: 19 Aug 2019First Look: 25 Jun 2019Accepted: 21 Jun 2019 Pages: 2665-2665 Information© 2019 The American Phytopathological SocietyFundingFunding Project for Science and Technology Planning Project under the Jurisdiction of Guangdong Entry-Exit Inspection and Quarantine BureauGrant/Award Number: 2018GDK64Keywordsbacterial diseaseDickeya fangzhongdaiPhalaenopsis aphroditesoft rotThe author(s) declare no conflict of interest.Cited byLoop-mediated isothermal amplification (LAMP) assay for specific and rapid detection of Dickeya fangzhongdai targeting a unique genomic region10 November 2022 | Scientific Reports, Vol. 12, No. 1Soft rot disease caused by Dickeya fangzhongdai in epiphytic orchids in Vietnam6 December 2021 | Canadian Journal of Plant Pathology, Vol. 44, No. 3Isolation and Genome Analysis of Pectobacterium colocasium sp. nov. and Pectobacterium aroidearum, Two New Pathogens of Taro26 April 2022 | Frontiers in Plant Science, Vol. 13First Report of Bacterial Soft Rot Disease on Taro Caused by Dickeya fangzhongdai in ChinaShufen Huang, Zhongqiao Chen, Ming Hu, Yang Xue, Lisheng Liao, and Lianhui Zhang15 November 2021 | Plant Disease, Vol. 105, No. 11Characterization of Dickeya fangzhongdai causing bacterial soft rot disease on Dendrobium nobile in India2 September 2020 | European Journal of Plant Pathology, Vol. 158, No. 3
Referência(s)