Artigo Acesso aberto Revisado por pares

The nucleolus functions as a phase-separated protein quality control compartment

2019; American Association for the Advancement of Science; Volume: 365; Issue: 6451 Linguagem: Inglês

10.1126/science.aaw9157

ISSN

1095-9203

Autores

Frédéric Frottin, Florian Schueder, Shivani Tiwary, Rajat Gupta, Roman Körner, Thomas Schlichthaerle, Jüergen Cox, Ralf Jungmann, F. Ulrich Hartl, Mark S. Hipp,

Tópico(s)

Fungal and yeast genetics research

Resumo

Phasing-in quality control in the nucleus The fundamental process of protein quality control in the nucleus is not well understood. The nucleus contains several non–membrane-bound subcompartments forming liquid-like condensates. The largest of these is the nucleolus, the site of ribosome biogenesis. Frottin et al. found that metastable nuclear proteins that misfold upon heat stress enter the nucleolus. In the nucleolus, they avoid irreversible aggregation and remain competent for heat shock protein 70–dependent refolding upon recovery from stress. Prolonged stress or the uptake of proteins associated with neurodegenerative diseases prevented this reversibility. Thus, the properties of a phase-separated compartment can assist in protein quality control. Science , this issue p. 342

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