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Maize Stalk Rot Caused by Fusarium falciforme (FSSC 3 + 4) in Mexico

2019; American Phytopathological Society; Volume: 103; Issue: 11 Linguagem: Inglês

10.1094/pdis-05-19-1055-pdn

1943-7692

A. Douriet-Angulo, Carlos Alfonso López‐Orona, Guadalupe Alfonso López-Urquídez, T. A. Vega-Gutiérrez, M. A. Tirado-Ramírez, Mitzi Estrada-Acosta, Felipe Ayala Tafoya, Moisés Gilberto Yáñez Juárez,

Plant Disease Resistance and Genetics

HomePlant DiseaseVol. 103, No. 11Maize Stalk Rot Caused by Fusarium falciforme (FSSC 3 + 4) in Mexico PreviousNext DISEASE NOTES OPENOpen Access licenseMaize Stalk Rot Caused by Fusarium falciforme (FSSC 3 + 4) in MexicoA. Douriet-Angulo, C. A. López-Orona, G. A. López-Urquídez, T. A. Vega-Gutiérrez, M. A. Tirado-Ramírez, M. D. Estrada-Acosta, F. Ayala-Tafoya, and M. G. Yáñez-JuárezA. Douriet-AnguloFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, C. A. López-Orona†Corresponding author: C. A. López-Orona; E-mail Address: clopezorona@uas.edu.mxhttp://orcid.org/0000-0003-2603-5562Facultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, G. A. López-UrquídezFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, T. A. Vega-GutiérrezFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, M. A. Tirado-RamírezFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, M. D. Estrada-AcostaFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, F. Ayala-TafoyaFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México, and M. G. Yáñez-JuárezFacultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, MéxicoAffiliationsAuthors and Affiliations A. Douriet-Angulo C. A. López-Orona † G. A. López-Urquídez T. A. Vega-Gutiérrez M. A. Tirado-Ramírez M. D. Estrada-Acosta F. Ayala-Tafoya M. G. Yáñez-Juárez Facultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México Published Online:13 Sep 2019https://doi.org/10.1094/PDIS-05-19-1055-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Stalk rot caused by Fusarium spp. is one of the most destructive diseases in maize-growing regions worldwide. This disease causes damage to the vascular bundles and may affect absorption and translocation of water and nutrients, thereby reducing photosynthesis and grain development and resulting in premature plant death (Li et al. 2010). Maize plants showing necrotic regions of the internal stalk tissues were collected from a field in Culiacan, Sinaloa, Mexico, in May 2018. Thirty samples were surface disinfested in 75% ethanol for 20 s and 0.3% NaClO for 2 min, rinsed with sterile distilled water, and cultured on potato dextrose agar (PDA). After incubation for 5 days at 28°C in the dark, fungal colonies with morphological characteristics of Fusarium spp. were observed. Monosporic isolates were obtained by transferring single spores to PDA and identified to species level based on morphological characteristics and DNA sequence analysis of translation elongation factor-1α (EF1-α) gene. White to cream-colored aerial mycelium of Fusarium colonies was observed on all cultures on PDA (Leslie and Summerell 2006). From 10-day-old cultures grown on carnation leaf agar medium, macroconidia were falciform, hyaline, with four to five septa, and measured 41.6 to 55.7 × 6.5 to 8.1 μm (n = 50); microconidia were hyaline, unicellular, oblong, with zero to two septa, measured 9.6 to 14.9 × 4.0 to 6.3 µm (n = 50), and were borne in false heads that measured 7.8 to 16.9 × 2.1 to 4.8 μm (n = 50); chlamydospores were not observed. The EF1-α gene was amplified by PCR and sequenced using the primers EF-1/EF-2 (O'Donnell et al. 1998). The sequence from the isolate C4M20T was deposited in GenBank (accession no. MK887202). Phylogenetic analysis by the neighbor-joining method was carried out using the EF1-α sequence (MK887202) from the C4M20T isolate and other species from the Fusarium solani species complex (FSSC). Phylogenetic analysis revealed the isolate was most closely related to Fusarium falciforme (FSSC 3 + 4) (100% bootstrap). The molecular identification was confirmed via BLAST on the FUSARIUM ID and Fusarium MLST databases. Pathogenicity tests with isolate C4M20T were done by individually inoculating 30 ten-leaf-stage maize plants at the second or third internode above the soil line using 20 μl of conidial suspension at a concentration of 106/ml as described by Zhang et al. (2016). Ten maize plants inoculated with sterilized water were used as a control. Plants were maintained for 60 days under greenhouse conditions with a 12-h photoperiod at 22 to 26°C and 70% relative humidity. After 20 days, all inoculated plants developed internal dark brown necrotic regions around the inoculated site. No symptoms were observed on the control plants after 60 days. The assay was conducted twice. The pathogen was reisolated from the necrotic tissue from inoculated plants and was identified by sequencing the partial EF1-α gene again as F. falciforme (FSSC 3 + 4) (O'Donnell et al. 2008). Although FSSC has been previously reported in maize causing ear rot in Estado de México, Mexico (Morales-Rodríguez et al. 2007) and Malaysia (Mohd Zainudin et al. 2017), to our knowledge, this is the first report of maize stalk rot caused by F. falciforme in Mexico. Therefore, further studies should focus on the epidemiology to evaluate the impact of the disease on yield and identify the risk factors.The author(s) declare no conflict of interest.References:Leslie, J. F., and Summerell, B. A., eds. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Oxford, U.K. https://doi.org/10.1002/9780470278376 Crossref, Google ScholarLi, W. J., et al. 2010. Agric. Sci. China 9:1467. https://doi.org/10.1016/S1671-2927(09)60239-X Crossref, Google ScholarMohd Zainudin, N. A. I., et al. 2017. Turk. J. Biol. 41:220. https://doi.org/10.3906/biy-1606-25 Crossref, ISI, Google ScholarMorales-Rodríguez, I., et al. 2007. Mycophatologia 163:31. https://doi.org/10.1007/s11046-006-0082-1 Crossref, ISI, Google ScholarO'Donnell, K., et al. 1998. PNAS 95:2044. https://doi.org/10.1073/pnas.95.5.2044 Crossref, ISI, Google ScholarO'Donnell, K., et al. 2008. J. Clin. Microbiol. 46:2477. https://doi.org/10.1128/JCM.02371-07 Crossref, ISI, Google ScholarZhang, Y., et al. 2016. PLoS Pathog 12:e1005485. https://doi.org/10.1371/journal.ppat.1005485 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Thanks are extended to Consejo Nacional de Ciencia y Tecnología for the grant 474532 for Master's studies to A. Douriet-Angulo.DetailsFiguresLiterature CitedRelated Vol. 103, No. 11 November 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionAdvanced symptoms of bacterial blotch disease on mushroom caps (Osdaghi et al.). Photo credit: C. Bull. 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Metrics Article History Issue Date: 4 Nov 2019Published: 13 Sep 2019First Look: 12 Jul 2019Accepted: 11 Jul 2019 Page: 2951 Information© 2019 The American Phytopathological SocietyFundingConsejo Nacional de Ciencia y TecnologíaGrant/Award Number: 474532KeywordsFusarium falciformemaizestalk rotThe author(s) declare no conflict of interest.Cited byIdentification of Pathogens and Evaluation of Resistance and Genetic Diversity of Maize Inbred Lines to Stalk Rot in Heilongjiang Province, ChinaJinxin Liu, Yujun Han, Wenqi Li, Tiancong Qi, Jiuming Zhang, and Yonggang Li17 February 2023 | Plant Disease, Vol. 107, No. 2Species Diversity in the Fusarium solani (Neocosmospora) Complex and Their Pathogenicity for Plants and Humans13 February 2023 | Doklady Biological Sciences, Vol. 507, No. 1Development of an Inoculation Technique for Rapidly Evaluating Maize Inbred Lines for Resistance to Stalk Rot caused by Fusarium spp. in the FieldWanyi Jiang, Wenbo Han, Ruiting Wang, Yonggang Li, Guanghui Hu, Jianfei Yang, Dan Jiang, Wenhao Han, Mingquan Wang, and Guoliang Li29 October 2021 | Plant Disease, Vol. 105, No. 9First Report of Fusarium falciforme (FSSC 3+4) Causing Wilt Disease of Phaseolus vulgaris in MexicoJosé F. 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