Portal de Periódicos da CAPES

Maternal fructose–induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi

2019; Wiley; Volume: 33; Issue: 10 Linguagem: Inglês

10.1096/fj.201901072r

1530-6860

Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Genki Mizuno, Nao Sadamoto, Yoshitaka Ando, Ryosuke Fujii, Kazuya Shiogama, Hiroaki Ishikawa, Koji Suzuki, Yohei Shimono, Koji Ohashi, Shuji Hashimoto,

Folate and B Vitamins Research

The FASEB JournalVolume 33, Issue 10 p. 11431-11442 ResearchFree to Read Maternal fructose–induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi Hiroya Yamada, Corresponding Author Hiroya Yamada hyamada@fujita-hu.ac.jp Department of Hygiene, Fujita Health University, Toyoake, JapanThese authors contributed equally to this work. Correspondence: Department of Hygiene, Fujita Health University School of Medicine, 1-98, Toyoake 470-1192, Japan. E-mail: hyamada@fujita-hu.ac.jpSearch for more papers by this authorEiji Munetsuna, Eiji Munetsuna Department of Biochemistry, Fujita Health University, Toyoake, JapanThese authors contributed equally to this work.Search for more papers by this authorMirai Yamazaki, Mirai Yamazaki Department of Clinical Biochemistry, Fujita Health University, Toyoake, Japan Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, JapanSearch for more papers by this authorGenki Mizuno, Genki Mizuno Joint Research Laboratory of Clinical Medicine, Fujita Health University, Toyoake, JapanSearch for more papers by this authorNao Sadamoto, Nao Sadamoto Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorYoshitaka Ando, Yoshitaka Ando Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorRyosuke Fujii, Ryosuke Fujii Department of Preventive Medical Sciences, Fujita Health University, Toyoake, JapanSearch for more papers by this authorKazuya Shiogama, Kazuya Shiogama Department of Clinical Examination, Fujita Health University School of Medical Sciences, Fujita Health University, Toyoake, JapanSearch for more papers by this authorHiroaki Ishikawa, Hiroaki Ishikawa Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorKoji Suzuki, Koji Suzuki Department of Preventive Medical Sciences, Fujita Health University, Toyoake, JapanSearch for more papers by this authorYohei Shimono, Yohei Shimono Department of Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorKoji Ohashi, Koji Ohashi Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorShuji Hashimoto, Shuji Hashimoto Department of Hygiene, Fujita Health University, Toyoake, JapanSearch for more papers by this author Hiroya Yamada, Corresponding Author Hiroya Yamada hyamada@fujita-hu.ac.jp Department of Hygiene, Fujita Health University, Toyoake, JapanThese authors contributed equally to this work. Correspondence: Department of Hygiene, Fujita Health University School of Medicine, 1-98, Toyoake 470-1192, Japan. E-mail: hyamada@fujita-hu.ac.jpSearch for more papers by this authorEiji Munetsuna, Eiji Munetsuna Department of Biochemistry, Fujita Health University, Toyoake, JapanThese authors contributed equally to this work.Search for more papers by this authorMirai Yamazaki, Mirai Yamazaki Department of Clinical Biochemistry, Fujita Health University, Toyoake, Japan Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, JapanSearch for more papers by this authorGenki Mizuno, Genki Mizuno Joint Research Laboratory of Clinical Medicine, Fujita Health University, Toyoake, JapanSearch for more papers by this authorNao Sadamoto, Nao Sadamoto Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorYoshitaka Ando, Yoshitaka Ando Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorRyosuke Fujii, Ryosuke Fujii Department of Preventive Medical Sciences, Fujita Health University, Toyoake, JapanSearch for more papers by this authorKazuya Shiogama, Kazuya Shiogama Department of Clinical Examination, Fujita Health University School of Medical Sciences, Fujita Health University, Toyoake, JapanSearch for more papers by this authorHiroaki Ishikawa, Hiroaki Ishikawa Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorKoji Suzuki, Koji Suzuki Department of Preventive Medical Sciences, Fujita Health University, Toyoake, JapanSearch for more papers by this authorYohei Shimono, Yohei Shimono Department of Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorKoji Ohashi, Koji Ohashi Department of Clinical Biochemistry, Fujita Health University, Toyoake, JapanSearch for more papers by this authorShuji Hashimoto, Shuji Hashimoto Department of Hygiene, Fujita Health University, Toyoake, JapanSearch for more papers by this author First published: 17 July 2019 https://doi.org/10.1096/fj.201901072RCitations: 2 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat ABSTRACT Fructose consumption is rising globally, but maternal high fructose intake might adversely affect offspring. Our previous report demonstrated that excess maternal fructose intake impairs hippocampal function in offspring, indicating that the hippocampi of offspring are highly sensitive to maternal fructose. Here, we examined the effect of maternal high fructose on mitochondrial physiology and uncoupling protein (UCP) expression. Rat dams received a 20% fructose solution during gestation and lactation. Immediately after weaning, offspring hippocampi were isolated. Maternal high fructose consumption attenuated the mitochondrial O2 consumption rate and stimulated lipid hydroperoxide production in the hippocampi of offspring. Reduced Ucp5 and mitochondrial transcription factor A (Tfam) mRNA levels were also observed after maternal exposure to fructose. We assessed the promoter regions of both genes and found that this treatment enhanced DNA methylation levels. In addition, luciferase assays showed that this DNA methylation could reduce the transcription of both genes. Chromatin immunoprecipitation analysis demonstrated that specificity protein 1 binding to the Ucp5 promoter regions was reduced by DNA methylation. In addition, Ucp5 knockdown induced the up-regulation of reactive oxygen species levels in a rat brain glioma cell line, whereas reduced O2 consumption was observed with Tfam knockdown. Maternal high fructose intake thus induces reduced O2 oxygen consumption and increases oxidative stress in offspring, at least partly through epigenetic mechanisms involving Ucp5 and Tfam.—Yamada, H., Munetsuna, E., Yamazaki, M., Mizuno, G., Sadamoto, N., Ando, Y., Fujii, R., Shiogama, K., Ishikawa, H., Suzuki, K., Shimono, Y., Ohashi, K., Hashimoto, S. Maternal fructose-induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi. FASEB J. 33, 11431–11442 (2019). www.fasebj.org Citing Literature Volume33, Issue10October 2019Pages 11431-11442 RelatedInformation