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BIBTEX RIS

Oral bacteriome of HIV‐1‐infected children from Rio de Janeiro, Brazil: Next‐generation DNA sequencing analysis

2019; Wiley; Volume: 46; Issue: 12 Linguagem: Inglês

10.1111/jcpe.13176

1600-051X

Lúcio Souza Gonçalves, Dennis de Carvalho Ferreira, Nicholas C. K. Heng, Fábio Vidal, Henrique Fragoso dos Santos, Diogo Godoy Zanicotti, Mariana M. Vasconcellos, Mayra Stambovsky, Blair Lawley, Norma de Paula Motta Rubini, Kátia Regina Netto dos Santos, G. J. Seymour,

Oral and gingival health research

This study compared the oral bacteriome between HIV-1-infected and non-HIV-1-infected Brazilian children/teenagers.Whole saliva, biofilm from the dorsal surface of the tongue and biofilm from supragingival and subgingival sites were collected from 27 HIV-1-infected and 30 non-HIV-1-infected individuals. Bacterial genomic DNA was extracted and 16S rRNA genes were sequenced using next-generation sequencing technology (Ion Torrent).In the supragingival biofilm, the phylum Firmicutes and genus Streptococcus sp. were more frequent in HIV-1-infected (95% and 78%, respectively) than in non-HIV-1-infected individuals (40% and 24%, respectively). In the subgingival biofilm of HIV-infected participants, the relative abundance of the Veillonella sp. and Prevotella sp. genera were higher than in non-HIV-1-infected participants. On the tongue, the genera with greater relative abundance in HIV-1-infected individuals were Neisseria sp. (21%). In saliva, the difference of the genus Prevotella sp. between non-HIV-1-infected and HIV-1-infected individuals was 15% and 7%, respectively. The Chao index revealed an increase in the richness of both sub- and supragingival biofilms in the HIV-1-infected samples compared with non-HIV-1-infected samples.HIV-1-infected children/teenagers have a higher frequency of the phyla Firmicutes and genus Streptococcus, and their oral microbiome shows more complexity than that of non-HIV-1-infected children/teenagers.