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BIBTEX RIS

Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of Musca domestica (Diptera: Muscidae) fed with Bacillus thuringiensis var. kyushuensis

2019; Brazilian Society of Tropical Medicine; Volume: 52; Linguagem: Inglês

10.1590/0037-8682-0135-2019

ISSN

1678-9849

Autores

Lorrane de Andrade Pereira, Vítor dos Santos Baía Ferreira, Nahara de Souza Leite, Sandra Maria de Oliveira Souza, Margareth Maria de Carvalho Queiroz, Suzana Côrte‐Real, Viviane Zahner,

Tópico(s)

Forensic Entomology and Diptera Studies

Resumo

INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.

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