The roles of reactive oxygen species and antioxidants in cryopreservation
2019; Portland Press; Volume: 39; Issue: 8 Linguagem: Inglês
10.1042/bsr20191601
ISSN1573-4935
AutoresJia Soon Len, Wen Shuo Darius Koh, Shi‐Xiong Tan,
Tópico(s)Selenium in Biological Systems
ResumoAbstract Cryopreservation has facilitated advancement of biological research by allowing the storage of cells over prolonged periods of time. While cryopreservation at extremely low temperatures would render cells metabolically inactive, cells suffer insults during the freezing and thawing process. Among such insults, the generation of supra-physiological levels of reactive oxygen species (ROS) could impair cellular functions and survival. Antioxidants are potential additives that were reported to partially or completely reverse freeze-thaw stress-associated impairments. This review aims to discuss the potential sources of cryopreservation-induced ROS and the effectiveness of antioxidant administration when used individually or in combination.
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