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First Report of Bulb Rot on Lily Caused by Curvularia pseudobrachyspora in China

2019; American Phytopathological Society; Volume: 104; Issue: 1 Linguagem: Inglês

10.1094/pdis-05-19-0943-pdn

1943-7692

Huilan Zeng, Rungen Li, Qineng Lu, Decong Yan,

Plant Disease Resistance and Genetics

HomePlant DiseaseVol. 104, No. 1First Report of Bulb Rot on Lily Caused by Curvularia pseudobrachyspora in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Bulb Rot on Lily Caused by Curvularia pseudobrachyspora in ChinaHuilan Zeng, Rungen Li, Qineng Lu, and Decong YanHuilan Zenghttp://orcid.org/0000-0001-7723-0486Department of Horticulture, College of Life Science and Environmental Resource, Yichun University, Yichun 336000, Jiangxi Province, China, Rungen Li†Corresponding author: R. Li; E-mail Address: 13507058200@163.comDepartment of Horticulture, College of Life Science and Environmental Resource, Yichun University, Yichun 336000, Jiangxi Province, China, Qineng LuDepartment of Horticulture, College of Life Science and Environmental Resource, Yichun University, Yichun 336000, Jiangxi Province, China, and Decong YanDepartment of Horticulture, College of Life Science and Environmental Resource, Yichun University, Yichun 336000, Jiangxi Province, ChinaAffiliationsAuthors and Affiliations Huilan Zeng Rungen Li † Qineng Lu Decong Yan Department of Horticulture, College of Life Science and Environmental Resource, Yichun University, Yichun 336000, Jiangxi Province, China Published Online:6 Nov 2019https://doi.org/10.1094/PDIS-05-19-0943-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Lilium brownii var. viridulum (Liliaceae) is as an edible bulbiferous plant cultivated extensively in Wanzai County, Yichun, Jiangxi Province, China. Lily bulbs are usually stored at 1 to 2°C to extend the supply period for commercial production. Five to 10% of stored bulbs exhibited rotting symptoms in a commercial product in January 2017. Initial symptoms on the visibly rotted bulbs in storage included 1- to 2-cm-diameter water-soaked lesions. Lesions turned light brown, then black and gradually increased in size until encompassing the whole bulb. Four light-brown symptomatic bulbs in storage were selected as samples. Symptomatic scales from the bulbs were removed and cut into small pieces (1 × 1 cm). Symptomatic plant tissues were surface sterilized with 70% ethanol for 1 min and 0.1% HgCl2 for 30 s, washed three times with sterilized distilled water, embedded in potato dextrose agar (PDA), and incubated at 25°C under a 12-h photoperiod. When a fungal colony that originated from the scale reached ∼1-cm diameter, a hyphal tip was transferred to a new PDA plate to obtain a single colony. The hyphae selection step was repeated once more until a pure single isolate, YB3-9, was obtained. The isolate was cultivated on a PDA plate as described above. The fungal colony reached 70-mm diameter in 1 week. Colonies appeared pale brown and downy with a black underside. Conidiophores were straight to flexuous, septate, sometimes branched, brown, and geniculate, and they bore one to three spores apically. Conidia were three- to four-celled, smooth, brown, geniculate, fusiform, clavate, and 11.9 to 24.8 × 5.1 to 13.1 μm, which was similar to a report by Marin-Felix et al. (2017). Basal and apical cells were pale. Intermediate cells were brown, and the second cell from the base was usually darker and larger than the others. These characteristics matched well with the description of Curvularia (Iftikhar et al. 2016; Manamgoda et al. 2012). To confirm this identification, fresh mycelium was scraped from the surface of a colonized agar plate and transferred to the microcentrifuge tube to extract DNA. DNA was extracted from the four mycelium samples from the same isolate using a Genomic DNA Purification Kit (Tiangen, Beijing), and the sequences of the rDNA internal transcribed spacer (ITS) region, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, and translation elongation factor 1-α (EF-1α) gene were amplified. The primers were those of Manamgoda et al. (2012) and Tan et al. (2014). The cycling conditions started with an initial denaturation at 94°C for 3 min; 35 cycles of 30 s denaturation at 94°C, 30 s annealing at 55°C, and 30 s elongation at 72°C; and a final elongation at 72°C for 7 min. All the sequences were submitted to GenBank. The ITS (MH819562), GAPDH (MH822837), and EF1-α (MH822836) sequences matched Curvularia pseudobrachyspora strain CPC 28808 reported by Marin-Felix et al. (2017) with 99, 100, and 99% similarity, respectively. To confirm pathogenicity, 6-month-old healthy (nearly 2-cm diameter) bulbs were sprayed with a conidial suspension (1 × 106 conidia/ml). Inoculated bulbs were placed in a growth chamber at 25°C with a 12-h light/dark cycle. Symptoms were similar to those initially observed, and all samples developed rot by 15 days postinoculation. A similar inoculation and puncture procedure was performed on leaves of 30-day-old plants (seedlings), and identical results were observed. Four negative control bulbs and leaves were sprayed with distilled water. No infection or disease symptoms were observed in unpunctured bulbs or plants. The pathogen was reisolated from all inoculated plants and its identity confirmed by microscopic identification and sequencing of ITS, GAPDH, and EF1-α. Bulb rot in lilies can also be caused by pathogens such as Fusarium oxysporum, Penicillium brevicompactum, and P. fellutanum (Hahm et al. 2006). This is the first report of C. pseudobrachyspora causing harm to L. brownii var. viridulum in China.The author(s) declare no conflict of interest.References:Hahm, S.-S., et al. 2006. Res. Plant Dis. 12:20. https://doi.org/10.5423/RPD.2006.12.1.020 Crossref, Google ScholarIftikhar, S., et al. 2016. Plant Dis. 100:1013. https://doi.org/10.1094/PDIS-09-15-1066-PDN Link, ISI, Google ScholarManamgoda, D. S., et al. 2012. Fungal Divers. 56:131. https://doi.org/10.1007/s13225-012-0189-2 Crossref, ISI, Google ScholarMarin-Felix, Y., et al. 2017. Mycosphere. 8:1556. https://doi.org/10.5943/mycosphere/8/9/11 Crossref, ISI, Google ScholarTan, Y. P., et al. 2014. Australas. Plant Pathol. 43:589. https://doi.org/10.1007/s13313-014-0315-6 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: This work is supported by the Key Research and Development Program of Science and Technology Agency of Jiangxi Province (20161BBF60040).DetailsFiguresLiterature CitedRelated Vol. 104, No. 1 January 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionStrawberry plant showing leaf blight caused by Pantoea ananatis FANS-1 (Bajpai et al.). Photo credit: B. Prithiviraj. Plasmopara velutina causing downy mildew on Impatiens balsamina (R. M. Silva et al.). Photo credit: R. W. Barreto. Metrics Article History Issue Date: 2 Jan 2020Published: 6 Nov 2019First Look: 14 Aug 2019Accepted: 10 Aug 2019 Pages: 291-291 Information© 2020 The American Phytopathological SocietyFundingKey Research and Development Program of Science and Technology Agency of Jiangxi ProvinceGrant/Award Number: 20161BBF60040Keywordsbulb rotlilyCurvularia pseudobrachysporaThe author(s) declare no conflict of interest.Cited byFirst Report of Fusarium fujikuroi Causing Bulb Rot on Lilium lancifolium in ChinaDongzhen Fang, Jia Chen, Yi Cheng, Zhimin Li, Litao Guo, Tuhong Wang, Qiao Deng, Jianping Xu, and Chunsheng Gao19 September 2021 | Plant Disease, Vol. 105, No. 8Curvularia spicifera and Curvularia muehlenbeckiae causing leaf blight on Cunninghamia lanceolata13 May 2020 | Plant Pathology, Vol. 69, No. 6