Portal de Periódicos da CAPES

Drosophila Snazarus Regulates a Lipid Droplet Population at Plasma Membrane-Droplet Contacts in Adipocytes

2019; Elsevier BV; Volume: 50; Issue: 5 Linguagem: Inglês

10.1016/j.devcel.2019.07.021

1878-1551

Rupali Ugrankar, Jade Bowerman, Hanaa Hariri, Mintu Chandra, Kai‐En Chen, Marie-France Bossanyi, Sanchari Datta, Sean Rogers, Kaitlyn M. Eckert, Gonçalo Vale, Alexia Victoria, Joseph Fresquez, Jeffrey G. McDonald, Steve Jean, Brett M. Collins, W. Mike Henne,

Photosynthetic Processes and Mechanisms

Adipocytes store nutrients as lipid droplets (LDs), but how they organize their LD stores to balance lipid uptake, storage, and mobilization remains poorly understood. Here, using Drosophila fat body (FB) adipocytes, we characterize spatially distinct LD populations that are maintained by different lipid pools. We identify peripheral LDs (pLDs) that make close contact with the plasma membrane (PM) and are maintained by lipophorin-dependent lipid trafficking. pLDs are distinct from larger cytoplasmic medial LDs (mLDs), which are maintained by FASN1-dependent de novo lipogenesis. We find that sorting nexin CG1514 or Snazarus (Snz) associates with pLDs and regulates LD homeostasis at ER-PM contact sites. Loss of SNZ perturbs pLD organization, whereas Snz over-expression drives LD expansion, triacylglyceride production, starvation resistance, and lifespan extension through a DESAT1-dependent pathway. We propose that Drosophila adipocytes maintain spatially distinct LD populations and identify Snz as a regulator of LD organization and inter-organelle crosstalk.