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First Report of Gummosis Disease of Sweet Cherry Caused by Botryosphaeria dothidea in China

2019; American Phytopathological Society; Volume: 103; Issue: 12 Linguagem: Inglês

10.1094/pdis-07-19-1418-pdn

1943-7692

Lizhen Zhang, Qibo Zhang, Pu Yang, Yu Niu, Wei Niu,

Forest Insect Ecology and Management

HomePlant DiseaseVol. 103, No. 12First Report of Gummosis Disease of Sweet Cherry Caused by Botryosphaeria dothidea in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Gummosis Disease of Sweet Cherry Caused by Botryosphaeria dothidea in ChinaL. Z. Zhang, Q. Zhang, P. Yang, Y. Niu, and W. NiuL. Z. Zhang†Corresponding author: L. Z. Zhang; E-mail Address: lizhen@sxu.edu.cnhttp://orcid.org/0000-0002-8418-4032School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China, Q. ZhangSchool of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China, P. YangSchool of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China, Y. NiuInstitute of Agricultural Resources and Economy, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, 030006, China, and W. NiuShanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, 030031, China AffiliationsAuthors and Affiliations L. Z. Zhang1 † Q. Zhang1 P. Yang1 Y. Niu2 W. Niu3 1School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China 2Institute of Agricultural Resources and Economy, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, 030006, China 3Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, 030031, China Published Online:20 Oct 2019https://doi.org/10.1094/PDIS-07-19-1418-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Prunus avium L. (sweet cherry) belongs to the Rosaceae family, genus Prunus, subspecies cerasus, and is widely grown in temperate-climate countries. In May 2018, sweet cherry gummosis on 2-year-old trees was found for more than 10% incidence of branches in an experimental orchard in Yuci City, Shanxi Province, China (37.68°N, 112.72°E). The sweet cherry breeding suffered –6°C freezing injury at flowering phase on April 6. Symptoms on infected trees appeared as green branches and axillary buds with tan discoloration initially. Later, slippery cortical cells swelled into thin seams, forming sunken and discolored lesions on nodes. Lenticels cracked with whitish or amber gummy exudation as well. Pathogen was isolated from infected twigs on potato dextrose agar (PDA) with 0.5% water extracts of sweet cherry twigs and incubated at 28°C for 4 days. Fungal colonies developed villous mycelium. No fungus was isolated from healthy trees. The front side of colonies on PDA initially was white and then turned gray and dark. The transparent mycelium had transverse septa and a smooth wall. Hyphae were branched and may have cross walls. The internal transcribed spacer region and the translation elongation factor 1-alpha were amplified and sequenced using primers ITS4/ITS5 and NS1/NS4, respectively (Lin and Wang 2019; Phillips et al. 2005). BLAST results showed 99.61 and 99.77% homology, respectively, with Botryosphaeria dothidea isolates from GenBank (accession nos. MK448265.1 and MH492625.1, and JX513624.1 and JX513622.1, respectively). Morphological and molecular results confirmed the isolate as B. dothidea (accession nos. SUB5881133 H-ITS5_A01.ab1MN104148 and BankIt2240954 H-EF_A01.ab1BotryosphaeriaMN138458). Pathogenicity testing was conducted on 1-year-old twigs of healthy sweet cherry trees in an orchard using a wound inoculation method. The experiments were performed twice, in June and in August, respectively. Prior to inoculation, single-spore isolates were grown on PDA at 28°C for 5 days. The 1-year-old twigs were surface disinfested with 70% ethanol for 30 s and then wounded by a sterilized needle. An approximately 5-mm-diameter mycelium-agar disc was placed on the wound. Similarly, other 1-year-old twigs were inoculated with a sterile quarter PDA disc on the needle wound as the negative control. All inoculated twigs were covered with sterile plastic film. One week after inoculation, all the inoculated twigs showed typical symptoms, and the gummy exudation appeared at the points of inoculation. No symptoms were observed on the negative controls, and the pathogenicity testing was repeated on three branches. The fungus was reisolated from all infected branches and was confirmed as B. dothidea based on morphological and molecular analyses, fulfilling Koch's postulates. In China, B. dothidea has been reported as the causal agent of gummosis disease of peach (Wang et al. 2011) and stem canker disease of Prunus serrulate (Yan et al. 2016). However, to the best of our knowledge, this is the first report of B. dothidea causing gummosis of sweet cherries.The author(s) declare no conflict of interest.References:Lin, H. J., and Wang, W. F. 2019. Plant Quar. 33:6. https://doi.org/10.19662/j.cnki.issn1005-2755.2019.03.003 Google ScholarPhillips, A. J. L., et al. 2005. Mycopathologia 159:433. https://doi.org/10.1007/s11046-005-0256-2 Crossref, ISI, Google ScholarWang, F., et al. 2011. Plant Dis. 95:1378. https://doi.org/10.1094/PDIS-12-10-0893 Link, ISI, Google ScholarYan, Y. C., et al. 2016. Plant Dis. 100:858. https://doi.org/10.1094/PDIS-09-15-0981-PDN Link, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Shanxi Provincial Foundation for Research and Development (grant no. 201803D221008-9).DetailsFiguresLiterature CitedRelated Vol. 103, No. 12 December 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionChlorotic symptom of Paris polyphylla var. yunnanensis infected by PMMoV-QJ (Wen et al.). Photo credit: M. F. Zhao. Symptoms of Puccinia triticina on wheat (Brar et al.). Photo credit: G. S. Brar. Metrics Downloaded 3,949 times Article History Issue Date: 21 Nov 2019Published: 20 Oct 2019First Look: 8 Aug 2019Accepted: 6 Aug 2019 Pages: 3283-3283 Information© 2019 The American Phytopathological SocietyFundingShanxi Provincial Foundation for Research and DevelopmentGrant/Award Number: 201803D221008-9Keywordsgummosis diseasesweet cherryBotryosphaeria dothideaChinaThe author(s) declare no conflict of interest.Cited ByThe heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits27 July 2022 | Frontiers in Microbiology, Vol. 13Endophytic Bacterium Serratia plymuthica From Chinese Leek Suppressed Apple Ring Rot on Postharvest Apple Fruit3 March 2022 | Frontiers in Microbiology, Vol. 12Single Nucleotide Polymorphism Detection for Peach Gummosis Disease Resistance by Genome-Wide Association Study7 February 2022 | Frontiers in Plant Science, Vol. 12Efficacy of Dimethyl Trisulfide on the Suppression of Ring Rot Disease Caused by Botryosphaeria dothidea and Induction of Defense-Related Genes on Apple Fruits7 February 2022 | Frontiers in Microbiology, Vol. 13