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Myrciaria tenella (DC.) O. Berg (Myrtaceae) Leaves as a Source of Antioxidant Compounds

2019; Multidisciplinary Digital Publishing Institute; Volume: 8; Issue: 8 Linguagem: Inglês

10.3390/antiox8080310

2076-3921

Ana Raquel Carneiro Ribeiro, Maria Lúcia da Silva Cordeiro, Larissa Silva, Cesar Orlando Muñoz Cadavid, Ricardo Basílio de Oliveira Caland, Marília Medeiros Fernandes de Negreiros, Moacir Fernandes Queiroz, Jefferson da Silva Barbosa, Cícero Flávio Soares Aragão, Silvana Maria Zucolotto, Riva de Paula Oliveira, Hugo Alexandre Oliveira Rocha, Kátia Castanho Scortecci,

Essential Oils and Antimicrobial Activity

Myrciaria species are widely studied to identify their chemical composition and evaluate their biological activity. Since evidence supporting the potential antioxidant and antiproliferative activity of Myrciaria tenella is lacking, the aim of this work was to evaluate these activities in six different leaf extracts: hexane (CHE), chloroform (CCE), ethanolic (CEE), methanolic (CME), aqueous final (CFAE), and only aqueous (CAE). The presence of phenolic compounds, tannin, saponin, and ursolic acid was determined by thin layer chromatography (TLC). CEE, CME, and CFAE showed in vitro antioxidant activity at the initiation, propagation, and termination stages of oxidative damage. Moreover, no toxicity was observed in the 3T3 non-cancerous cell line. On the other hand, all extracts promoted cell death in the tumor cell lines human cervical adenocarcinoma cell line (HeLa) and human stomach gastric adenocarcinoma cell line (AGS). Based on these results, the effect of CEE on the AGS cell line was analyzed using flow cytometry, and necrosis and late apoptosis were observed. Finally, the Caenorhabditis elegans model showed that CEE was able to reduce the basal reactive oxygen species (ROS) level. Ultra-performance liquid chromatography (UPLC) analysis showed rutin as the major compound in CEE. Therefore, Myrciaria tenella fresh leaves may be potential sources of molecules possessing antioxidant and antiproliferative activities.