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Determination of serotonin by using a thin film containing graphite, nanodiamonds and gold nanoparticles anchored in casein

2019; Elsevier BV; Volume: 149; Linguagem: Inglês

10.1016/j.measurement.2019.106979

1873-412X

Matheus Mesquita Vidal Ramos, Jefferson H.S. Carvalho, Paulo Roberto de Oliveira, Bruno C. Janegitz,

Dye analysis and toxicity

The determination of serotonin has become relevant in biological fluid analysis since some neurological disorders may be related to abnormal amounts of this neurotransmitter in human beings. In this work, it is proposed the construction of a low-cost electrode sensitive to the quantification of serotonin by a biofilm of graphite, nanodiamonds and gold nanoparticles anchored in casein. The casein biofilm was used to anchor the conductive nanomaterials on the GCE. Measurements were performed by differential pulse voltammetry in which showed a linear response with LDR of 0.3 to 3.0 μmol L−1, LOD of 0.1 μmol L−1 and a sensitivity of 0.18 µA L mol−1. The sensor was applied for the serotonin quantification in synthetic urine samples enriched with good recovery (91.4 to 103%). Therefore, the proposed electrode improved the conductivity and the electronic transfer capacity of the pristine electrode by low-cost biofilm, which could be applied in electrochemical biosensing.