Voltar
Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Euterpe oleracea Mart. (Açai) Supplementation Attenuates Acute Doxorubicin-Induced Cardiotoxicity in Rats

2019; Karger Publishers; Volume: 53; Issue: 2 Linguagem: Inglês

10.33594/000000145

ISSN

1421-9778

Autores

Lívia Maria Beraldo Simões Mathias, Patricia Alegre, Isadora de Oliveira Fernandes dos Santos, Tatiana Fernanda Bachiega, Amanda Menezes Figueiredo, Fernanda Chiuso‐Minicucci, Ana Angélica Henrique Fernandes, Silméia Garcia Zanati Bazan, Marcos Ferreira Minicucci, Paula S. Azevedo, Marina Politi Okoshi, Zornoff Leonardo Antonio Mamede, Sérgio Alberto Rupp de Paiva, Bertha Furlan Polegato,

Tópico(s)

Cancer Treatment and Pharmacology

Resumo

Background/Aims: Doxorubicin, a chemotherapy drug used successfully for years, could induce cardiotoxicity.Euterpe oleracea Mart.(açai) is a fruit high in antioxidant properties.The aim of this study was to evaluate doxorubicin-induced cardiotoxicity prevention after açai administration.Methods: A total of 64 male Wistar rats were allocated into 4 groups: control (C), açai (A), doxorubicin (D) and açai-doxorubicin (DA).Rats received regular chow (C and D groups) or chow supplemented with açai 5% (A and DA groups) for 4 weeks.Subsequently, rats received doxorubicin 20 mg/kg (D and DA groups) or saline (C and A groups).Euthanasia was performed 48 hours after doxorubicin injection.Left ventricular function was evaluated by echocardiography in vivo and by isolated heart study ex vivo.Oxidative stress, myocardial metabolism and nitric oxide metabolite were evaluated by spectrophotometry, MMP-2 activity by zymography and caspase-3 and Bcl-2 protein expression by Western blot.Results: Doxorubicin induced decreases in body weight, food and water ingestion.We observed decreases in left ventricular fractional shortening in rats treated with doxorubicin.Additionally, the same rats showed lower +dP/dt and -dP/dt during isolated heart study than those who did not receive doxorubicin.Doxorubicin injection increased caspase-3 protein expression, myocardium lipid hydroperoxide concentration, MMP-2 activity, phosphofructokinase and lactate dehydrogenase activity, and decreased β-hydroxyacyl-CoA dehydrogenase, pyruvate dehydrogenase, citrate synthase, complex I, complex II and ATP synthase activity

Referência(s)