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Photobiomodulation Enhances Cisplatin Cytotoxicity in a Culture Model with Oral Cell Lineages

2019; Wiley; Volume: 96; Issue: 1 Linguagem: Inglês

10.1111/php.13152

1751-1097

Ivana Márcia Alves Diniz, Giovanna Ribeiro Souto, Iuri Dornelas Prates Freitas, José Alcides Almeida de Arruda, Janine Mayra da Silva, Tarcı́lia Aparecida Silva, Ricardo Alves Mesquita,

Dermatologic Treatments and Research

Abstract Cisplatin plays a central role in cancer chemotherapy, but resistance to this drug remains a major obstacle in treatment. Drawbacks related to cisplatin failure may be associated with cell energy metabolism. This study investigated whether photobiomodulation ( PBM ) can potentiate the effects of cisplatin on keratinocytes (HaCat) and cancer cells ( SCC 25 and HN 12). Cells were treated with laser (GaAlAs; 660 nm; 60 mW; 0.33 J; 2.14 W cm −2 ; 11.7 J cm −2 and 6 s) and cisplatin (7.8 μg mL −1 ) to evaluate cell viability, Ki‐67, VEGF , TGF ‐ β 1, EGF expression and ROS production. Observations were validated in the SCC 25 cell lineage, where the type of cell death (necrosis/apoptosis) and the amount of ATP were assessed. Cell lineages showed increased sensitivity to cisplatin associated with PBM (Cis‐ PBM ). Ki‐67 was augmented in all cell lineages treated with Cis‐ PBM when compared to cisplatin alone (Cis). Cis or Cis‐ PBM significantly decreased VEGF expression in cancer cells, while no changes were seen in the expression of TGF ‐ β 1 or EGF compared to control. ROS levels were similar in the Cis and Cis‐ PBM groups. Cells treated with Cis‐ PBM died by apoptosis, leading to greater consumption of ATP . These observations suggest that PBM may potentiate the effects of cisplatin, leading to increased drug cytotoxicity and enhanced cell death.