Produção Nacional
Revisado por pares
Metabolic Profile of Supragingival Plaque Exposed to Arginine and Fluoride
2019; SAGE Publishing; Volume: 98; Issue: 11 Linguagem: Inglês
10.1177/0022034519869906
ISSN1544-0591
AutoresMarcelle M. Nascimento, Andres Alvarez, X. L. Huang, C. M. Browngardt, R. Jenkins, M.C. Sinhoreti, Ana Paula Dias Ribeiro, Deborah A. Dilbone, Vincent P. Richards, Timothy J. Garrett, Robert A. Burne,
Tópico(s)Dental Health and Care Utilization
ResumoCaries lesions develop when acid production from bacterial metabolism of dietary carbohydrates outweighs the various mechanisms that promote pH homeostasis, including bacterial alkali production. Therapies that provide arginine as a substrate for alkali production in supragingival oral biofilms have strong anticaries potential. The objective of this study was to investigate the metabolic profile of site-specific supragingival plaque in response to the use of arginine (Arg: 1.5% arginine, fluoride-free) or fluoride (F: 1,100 ppm F/NaF) toothpastes. Eighty-three adults of different caries status were recruited and assigned to treatment with Arg or F for 12 wk. Caries lesions were diagnosed using International Caries Detection and Assessment System II, and plaque samples were collected from caries-free and carious tooth surfaces. Taxonomic profiles were obtained by HOMINGS (Human Oral Microbe Identification using Next Generation Sequencing), and plaque metabolism was assessed by the levels of arginine catabolism via the arginine deiminase pathway (ADS), acidogenicity, and global metabolomics. Principal component analysis (PCA), partial least squares-discriminant analysis, analysis of variance, and random forest tests were used to distinguish metabolic profiles. Of the 509 active lesions diagnosed at baseline, 70 (14%) were inactive after 12 wk. Generalized linear model showed that enamel lesions were significantly more likely to become inactive compared to dentin lesions (
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