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Enhanced NLRP3 and DEFA1B Expression During the Active Stage of Parenchymal Neuro-Behçet's Disease

2019; Stanford University Highwire Press; Volume: 33; Issue: 5 Linguagem: Inglês

10.21873/invivo.11629

1791-7549

Elif Uğurel, Ece Erdağ, Cem İ̇smail Küçükali, AYCA OLCAY, Elif Şanlı, Ece Akbayır, Murat Kürtüncü, Tuncay Gündüz, Vuslat Yılmaz, Erdem Tüzün, Burçak Vural,

Autoimmune and Inflammatory Disorders Research

Neurological symptoms (neuro-Behçet's disease; NBD) occur in a fraction of Behçet's disease (BD) patients and often present with parenchymal brain lesions and clinical exacerbations. Our aim was to identify genes associated with attack and remission periods of NBD.Microarray analysis was performed using peripheral blood mononuclear cell (PBMC) samples obtained during attack and remission periods of five NBD patients. Expression levels of the most significantly up-regulated genes were measured with real-time PCR using PBMC samples of 15 NBD patients and 20 healthy controls.During NBD attacks, the most remarkably up-regulated genes were defensin alpha 1B (DEFA1B) and NLR family, pyrin domain containing 3 (NLRP3). Real time PCR studies showed significantly increased DEFA1B and NLRP3 expression levels during attacks.Immunological factors showing the most significant increase in expression during NBD attacks were primarily associated with innate immunity functions. DEFA1B and NLRP3 can be used as biomarkers for estimation of disease activity in NBD.