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First Report of Diaporthe kongii Causing Foot Rot on Sweet Potato in Brazil
2019; American Phytopathological Society; Volume: 104; Issue: 1 Linguagem: Inglês
10.1094/pdis-04-19-0868-pdn
ISSN1943-7692
AutoresThaís Regina Pintino De Almeida, I. L. Coelho, Leirson Salvador Bezerra de Vasconcelos, Maria Aparecida Pontes, Willie Anderson dos Santos Vieira, Marcos Paz Saraiva Câmara, Vinson P. Doyle, Delson Laranjeira,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 104, No. 1First Report of Diaporthe kongii Causing Foot Rot on Sweet Potato in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Diaporthe kongii Causing Foot Rot on Sweet Potato in BrazilT. R. P. Almeida, I. L. Coelho, L. S. B. Vasconcelos, M. A. Pontes, W. A. S. Vieira, M. P. S. Câmara, V. P. Doyle, and D. LaranjeiraT. R. P. Almeida†Corresponding author: T. R. P. Almeida; E-mail Address: thais_regina321@hotmail.comhttp://orcid.org/0000-0003-2519-3710Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil, I. L. CoelhoDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil, L. S. B. VasconcelosInstituto Agronômico de Pernambuco, Camocim de São Félix, 55665-000, Pernambuco, Brazil, M. A. PontesSecretaria Agricultura, Meio Ambiente e Recursos Hídricos de São Joaquim do Monte, São Joaquim do Monte, 57770-000, Pernambuco, Brazil, W. A. S. VieiraDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil, M. P. S. Câmarahttp://orcid.org/0000-0002-7930-7886Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil, V. P. DoyleDepartment of Plant Pathology and Crop Physiology, Louisiana State University AgCenter, Baton Rouge, LA, U.S.A., and D. Laranjeirahttp://orcid.org/0000-0002-1895-1766Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil AffiliationsAuthors and Affiliations T. R. P. Almeida1 † I. L. Coelho1 L. S. B. Vasconcelos2 M. A. Pontes3 W. A. S. Vieira1 M. P. S. Câmara1 V. P. Doyle4 D. Laranjeira1 1Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil 2Instituto Agronômico de Pernambuco, Camocim de São Félix, 55665-000, Pernambuco, Brazil 3Secretaria Agricultura, Meio Ambiente e Recursos Hídricos de São Joaquim do Monte, São Joaquim do Monte, 57770-000, Pernambuco, Brazil 4Department of Plant Pathology and Crop Physiology, Louisiana State University AgCenter, Baton Rouge, LA, U.S.A. Published Online:11 Nov 2019https://doi.org/10.1094/PDIS-04-19-0868-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sweet potatoes (Ipomoea batatas L.) are one of the most important crops in the world, especially in developing countries. Foot rot of sweet potato is one of the major diseases responsible for economic losses in the crop, making some areas of production unviable. Reports show that foot rot of sweet potato causes losses of 80% in the productivity of the cultivar CO-BRANCA in the area. Postharvest rot may occur on storage roots, making them unviable for commercialization. In May 2017, symptoms similar to foot rot, such as brown to black lesions on roots and yellowing of leaves, were observed in plants and roots of cultivar CO-BRANCA in Belém de Maria, Pernambuco, Brazil (8°32′41.7″S, 35°50′47.0″W) with a disease incidence of 50%. Fragments of symptomatic tissues were removed from the lesion transition area, surface disinfested (45 s in 70% alcohol, 1 min in 1% NaOCl, and rinsed twice in sterile water), and plated on potato dextrose agar (PDA). The representative isolate CFS 627, deposited in the Collection of Soil Fungi (UFRPE), produced white to grayish tufted aerial mycelium and a brown reverse with an isabelline ring and black stromatic pycnidia, morphologically similar to those of Diaporthe endophytica, D. masirevicii, and D. kongii (Gao et al. 2017; Thompson et al. 2011, 2015). The identity of the isolate was determined by maximum likelihood and Bayesian inference of the sequences of nrITS and EF1-α regions, GenBank accession numbers MK840849 and MK829788. The isolate shared 99% identity (E value = 0.0) and 98.8% identity (E value = 0.0) with nrITS (NR111616) and EF1-α (JN645797) sequences, respectively, from the ex-type of D. kongii BRIP 54031 and was nested within the clade that includes D. kongii isolates with 99% bootstraps support and 1.0 Bayesian inference. The pathogenicity of CFS 627 isolate was confirmed by inoculation using mycelial plugs from 7-day-old cultures grown on PDA. Each storage root was punctured with a needle five times in two locations using a hypodermic needle (13 × 4.5 mm), and a mycelial plug was placed in contact with the host tissue at each location (four replicates). The storage roots inoculated with sterile PDA plugs represented the negative control. The inoculated sweet potatoes were kept in a humid chamber, at 12-h photoperiod, for 48 h (27 ± 2°C and 75% relative humidity). The storage roots inoculated with CFS 627 showed symptoms of rot, producing brown lesions that became blackened, similar to the symptoms observed in the field, whereas the controls remained asymptomatic. The pathogens were reisolated, and the fungal colonies recovered were similar to CFS 627 in morphology, thus fulfilling Koch's postulates. D. kongii was previously reported in association with sunflower, peanut, and Portulaca grandiflora in Australia (Thompson et al. 2011, 2018). To the best of our knowledge, this is the first report of D. kongii causing disease on sweet potatoes in the world. The identification of new fungal species pathogenic to sweet potato contributes to the choice of appropriate measures for the management of the pathogen, reducing its action on the crop. Therefore, future research is necessary to develop efficient phytosanitary management strategies for this pathogen.The author(s) declare no conflict of interest.References:Gao, Y., et al. 2017. Syst. Biodivers. 14:1. https://doi.org/10.5598/imafungus.2017.08.01.11 Google ScholarThompson, S. M., et al. 2011. Persoonia 27:80. https://doi.org/10.3767/003158511X617110 Crossref, ISI, Google ScholarThompson, S. M., et al. 2015. Persoonia 35:39. https://doi.org/10.3767/003158515X687506 Crossref, ISI, Google ScholarThompson, S. M., et al. 2018. Plant Dis. 102:1459. https://doi.org/10.1094/PDIS-12-17-1930-PDN Link, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 1 January 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionStrawberry plant showing leaf blight caused by Pantoea ananatis FANS-1 (Bajpai et al.). Photo credit: B. Prithiviraj. Plasmopara velutina causing downy mildew on Impatiens balsamina (R. M. Silva et al.). Photo credit: R. W. Barreto. Metrics Downloaded 2,912 times Article History Issue Date: 2 Jan 2020Published: 11 Nov 2019First Look: 5 Sep 2019Accepted: 2 Sep 2019 Pages: 284-284 Information© 2020 The American Phytopathological SocietyKeywordsIpomoea batatasfoot rotDiaporthe kongiistorage tuber rotThe author(s) declare no conflict of interest.Cited ByDiaporthe Seed Decay of Soybean [Glycine max (L.) Merr.] Is Endemic in the United States, But New Fungi Are InvolvedKristina Petrović, Demetra Skaltsas, Lisa A. 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