Unexplored BACTERIAL DIVERSITY OF Rajapur-Unhale HOT SPRING, Ratnagiri DISTRICT, Maharastra, India- A Gold STANDARD AND Maldi-Tof BASED APPROACH
2019; Volume: 7; Issue: 4 Linguagem: Inglês
10.32622/ijrat.742019110
ISSN2321-9637
AutoresRajendran Mathan Kumar, Suresh S. S. Raja,
Tópico(s)Mineral Processing and Grinding
ResumoIn our study sediment and water samples was collected from the Rajapur-Unhale hot spring having 42 O C temperature.This study aimed to explore the unexplored bacterial diversity within the Rajapur-Unhale hotsprings.Fifty five isolates were obtained from both sediment and water samples were subjected to MALDI-TOF identification, were able to identify 15 isolates and most of the isolates which was not identified by direct cell loading method but was identified using ethanol/ formic acid extraction method and others gave not reliable identification with both the methods.For identification of bacterial species and genera, the sequencing centered on 16S rRNA is most extensively used and accepted to categorize them phylogenetically.Whereas, 16S rRNA gene sequencing based identification could able to identify all the 55 isolates.This study shows the clear picture that the MALDI-TOF database needs to be updated with environmental isolates for effective and appropriate identification.
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