First Report of Fusarium oxysporum f. sp. vasinfectum Race 4 Causing Fusarium Wilt of Cotton in New Mexico, U.S.A.
2019; American Phytopathological Society; Volume: 104; Issue: 2 Linguagem: Inglês
10.1094/pdis-06-19-1170-pdn
ISSN1943-7692
AutoresYi Zhu, Phillip Lujan, T.C. Wedegaertner, R. L. Nichols, A. Abdelraheem, J. F. Zhang, Soum Sanogo,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 104, No. 2First Report of Fusarium oxysporum f. sp. vasinfectum Race 4 Causing Fusarium Wilt of Cotton in New Mexico, U.S.A. PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium oxysporum f. sp. vasinfectum Race 4 Causing Fusarium Wilt of Cotton in New Mexico, U.S.A.Y. Zhu, P. A. Lujan, T. Wedegaertner, R. Nichols, A. Abdelraheem, J. F. Zhang, and S. SanogoY. Zhuhttp://orcid.org/0000-0003-3556-2743Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM 88003, P. A. LujanDepartment of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM 88003, T. WedegaertnerCotton Incorporated, Cary, NC 27513, R. NicholsCotton Incorporated, Cary, NC 27513, A. AbdelraheemDepartment of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM 88003, J. F. Zhang†Corresponding authors: J. F. Zhang; E-mail Address: jinzhang@nmsu.edu and S. Sanogo; E-mail Address: ssanogo@nmsu.eduDepartment of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM 88003, and S. Sanogo†Corresponding authors: J. F. Zhang; E-mail Address: jinzhang@nmsu.edu and S. Sanogo; E-mail Address: ssanogo@nmsu.eduhttp://orcid.org/0000-0003-0065-3856Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM 88003 AffiliationsAuthors and Affiliations Y. Zhu1 P. A. Lujan2 T. Wedegaertner3 R. Nichols3 A. Abdelraheem1 J. F. Zhang1 † S. Sanogo2 † 1Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM 88003 2Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM 88003 3Cotton Incorporated, Cary, NC 27513 Published Online:19 Nov 2019https://doi.org/10.1094/PDIS-06-19-1170-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Fusarium wilt (FW), caused by the soil-borne pathogen Fusarium oxysporum f. sp. vasinfectum (FOV), is one of the most destructive diseases in cotton (Gossypium hirsutum and G. barbadense). Of the eight described races of FOV recognized worldwide, FOV race 4 is among the most virulent. FOV race 4 was first identified in India (Armstrong and Armstrong 1960). It was isolated from cotton in California in 2003 and in El Paso, Texas, in 2017 (Halpern et al. 2018; Kim et al. 2005). In June to September 2017 and 2018, about 1% of the cotton plants in two fields of Las Cruces and Berino, NM, displayed typical symptoms of FW, including leaf wilt, chlorosis, and vascular discoloration. To isolate the causal agent, root tissues from 10 symptomatic plants from each field were washed free of soil, surface disinfested in 0.8% sodium hypochlorite for 3 min, rinsed two to three times with sterile distilled water, and blotted dry with a paper towel. Five root segments (1 cm) were plated on acidified PDA medium (pH = 4.8) and incubated at 25°C for 5 days. A fungus morphologically consistent with Fusarium was isolated from the diseased root segments at an isolation frequency of approximately 70% in each field. Two single-spored isolates (one from each of two fields) were derived from colonies resembling Fusarium spp. and subcultured on PDA at 25°C for 7 to 10 days. The colonies each exhibited white floccose mycelium with violet pigmentation on PDA, characteristic of F. oxysporum (Leslie and Summerell 2006). Macroconidia were straight or slightly curved, with three septa, the curved apical cell sometimes with a slight hook, and a foot-shaped basal cell (19.5 to 39.8 × 3.9 to 5.0 μm, n = 30). Microconidia were oval to kidney shaped, aseptate, and were produced in false heads on short monophialides (5.3 to 10.8 × 2.1 to 4.1 μm, n = 30). Chlamydospores (8.2 to 14.1 μm in diameter, n = 10) were smooth-walled and formed singly or in pairs. Genomic DNA of the two isolates was extracted for amplification and sequencing of the fragments of the intergenic spacer (IGS) regions (GenBank nos. MK953693 and MK953694), translation elongation factor gene (EF-1α) (MK953689 and MK953690), and β-tubulin gene (MK953691 and MK953692) (Cianchetta et al. 2015). The sequences for the two isolates were identical to these from FOV race 4 reference isolate NRRL 25434 (IGS, accession no. FJ985475) and reference isolate ATCC 16613 (EF-1α, KT 323858; and β-tubulin gene, KT323817). A phylogenetic analysis based on the sequences clustered the two isolates with the FOV race 4 reference isolates. Additionally, PCR amplifications of the two isolates were positive using the FOV race 4-specific primers R4f/R4r (Yang et al. 2006). Thus, the two isolates were identified as FOV race 4. Pathogenicity of the two isolates was first tested on Pima (G. barbadense) 'DP 357' using a soil infestation method (Sanogo and Zhang 2016), and both isolates were found to cause chlorosis, wilting, and plant death on the inoculated plants, whereas the control plants grown in soil mixed with sterilized water remained healthy. To further identify whether the isolates can infect both Upland (G. hirsutum) and Pima cotton, one representative isolate was used to inoculate four cotton genotypes (Upland, Acala 1517-20 and NM 16M1234; Pima, PHY 841 RF and DP 358 RF) at the first true-leaf stage by pipetting 25 ml of a conidial suspension (1 × 106 conidia/ml) onto the soil around the base of the plants. In the control treatment, only sterilized water was used. The tests were performed twice with four replications (10 plants per genotype per replication). All plants were maintained in greenhouse at 25 to 28°C with a 12-h photoperiod. Similar to those observed in fields, leaf chlorosis, necrosis, wilting, and leaf abscissions were observed on all the genotypes 30 days after inoculation. The isolate was successfully reisolated from roots of the infected plants using the same method as described above. To our knowledge, this is the first written report with both morphological and DNA sequence information of FOV race 4 causing FW on Upland and Pima cotton in New Mexico. The results will assist in managing the disease on cotton in the region.The author(s) declare no conflict of interest.References:Armstrong, G. M., and Armstrong, J. K. 1960. U.S. Dep. Agric. Tech. Bull. 1219:1. Google ScholarCianchetta, A. N., et al. 2015. J. Cotton Sci. 19:328. ISI, Google ScholarHalpern, H. C., et al. 2018. Plant Dis. 102:446. https://doi.org/10.1094/PDIS-07-17-1084-PDN Link, Google ScholarKim, Y., et al. 2005. Plant Dis. 89:366. https://doi.org/10.1094/PD-89-0366 Link, ISI, Google ScholarLeslie, J. F., and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Oxford, U.K. doi.org/10.1002/9780470278376 Crossref, Google ScholarSanogo, S., and Zhang, J. 2016. Euphytica 207:255. https://doi.org/10.1007/s10681-015-1532-y Crossref, ISI, Google ScholarYang, M. E., et al. 2006. Page 93 in: Proc. Beltwide Cotton Conf., San Antonio, TX. Google ScholarY. Zhu and P. A. Lujan contributed equally.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 2 February 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptom of maize ear rot caused by Fusarium sporotrichioides (B. B. Wang et al.). Photo credit: C. X. Duan. Systemic symptoms of alfalfa mosaic virus (AMV) isolate CaM on leaves of potato (X. Z. Nie et al.). Photo credit: X. Z. Nie. Metrics Downloaded 2,631 times Article History Issue Date: 31 Jan 2020Published: 19 Nov 2019First Look: 18 Sep 2019Accepted: 12 Sep 2019 Pages: 588-588 Information© 2020 The American Phytopathological SocietyKeywordsFusarium wiltGossypium spp.FOV race 4The author(s) declare no conflict of interest.Cited ByDetection and Genotyping of Fov4 (Race 4, VCG0114), the Fusarium Wilt Pathogen of23 October 2021Detection and Characterization of Fusarium Wilt (Fusarium oxysporum f. sp. vasinfectum) Race 4 Causing Fusarium Wilt of Cotton Seedlings in New MexicoYi Zhu, Abdelraheem Abdelraheem, Phillip Lujan, John Idowu, Patrick Sullivan, Robert Nichols, Tom Wedegaertner, and Jinfa Zhang16 November 2021 | Plant Disease, Vol. 0, No. 0Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays5 November 2021 | Biology, Vol. 10, No. 11Diallel analysis of resistance to Fusarium wilt Race 4 in American pima cotton30 September 2021 | Crop Science, Vol. 61, No. 6Size‐exclusion chromatography allows the isolation of EVs from the filamentous fungal plant pathogen Fusarium oxysporum f. sp. vasinfectum (Fov)8 March 2021 | PROTEOMICS, Vol. 21, No. 13-14Identification of resistance sources to Fusarium wilt race 4 in Gossypium barbadense and cultivated Asiatic diploid species30 June 2021 | Euphytica, Vol. 217, No. 7Characterization of Current Fusarium oxysporum f. sp. vasinfectum Isolates from Cotton in the San Joaquin Valley of California and Lower Valley El Paso, TexasJosue Diaz, Jorge Garcia, Celeste Lara, Robert B. 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